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Transcriptome Analysis for Abnormal Spike Development of the Wheat Mutant dms
- Source :
- PLoS ONE, PLoS ONE, Vol 11, Iss 3, p e0149287 (2016)
- Publication Year :
- 2015
-
Abstract
- Background Wheat (Triticum aestivum L.) spike development is the foundation for grain yield. We obtained a novel wheat mutant, dms, characterized as dwarf, multi-pistil and sterility. Although the genetic changes are not clear, the heredity of traits suggests that a recessive gene locus controls the two traits of multi-pistil and sterility in self-pollinating populations of the medium plants (M), such that the dwarf genotype (D) and tall genotype (T) in the progeny of the mutant are ideal lines for studies regarding wheat spike development. The objective of this study was to explore the molecular basis for spike abnormalities of dwarf genotype. Results Four unigene libraries were assembled by sequencing the mRNAs of the super-bulked differentiating spikes and stem tips of the D and T plants. Using integrative analysis, we identified 419 genes highly expressed in spikes, including nine typical homeotic genes of the MADS-box family and the genes TaAP2, TaFL and TaDL. We also identified 143 genes that were significantly different between young spikes of T and D, and 26 genes that were putatively involved in spike differentiation. The result showed that the expression levels of TaAP1-2, TaAP2, and other genes involved in the majority of biological processes such as transcription, translation, cell division, photosynthesis, carbohydrate transport and metabolism, and energy production and conversion were significantly lower in D than in T. Conclusions We identified a set of genes related to wheat floral organ differentiation, including typical homeotic genes. Our results showed that the major causal factors resulting in the spike abnormalities of dms were the lower expression homeotic genes, hormonal imbalance, repressed biological processes, and deficiency of construction materials and energy. We performed a series of studies on the homeotic genes, however the other three causal factors for spike abnormal phenotype of dms need further study.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Metabolic Processes
UniGene
lcsh:Medicine
Gene Expression
01 natural sciences
Biochemistry
Transcriptome
lcsh:Science
Triticum
Genetics
Multidisciplinary
food and beverages
Agriculture
Plants
Phenotype
Wheat
Carbohydrate Metabolism
Metabolic Pathways
Homeotic gene
Research Article
Carbohydrate transport
Locus (genetics)
Crops
Biology
Genes, Plant
03 medical and health sciences
DNA-binding proteins
Gene Regulation
RNA, Messenger
Grasses
Gene
Gene Expression Profiling
lcsh:R
Organisms
Biology and Life Sciences
Proteins
Regulatory Proteins
Gene expression profiling
030104 developmental biology
Metabolism
Mutation
lcsh:Q
010606 plant biology & botany
Crop Science
Cereal Crops
Transcription Factors
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....4a720d0b8a00194a9b93a87a99afb2c5