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Elucidation of Exosome Migration across the Blood-Brain Barrier Model In Vitro
- Source :
- Cellular and molecular bioengineering
- Publication Year :
- 2016
-
Abstract
- The delivery of therapeutics to the central nervous system (CNS) remains a major challenge in part due to the presence of the blood-brain barrier (BBB). Recently, cell-derived vesicles, particularly exosomes, have emerged as an attractive vehicle for targeting drugs to the brain, but whether or how they cross the BBB remains unclear. Here, we investigated the interactions between exosomes and brain microvascular endothelial cells (BMECs) in vitro under conditions that mimic the healthy and inflamed BBB in vivo. Transwell assays revealed that luciferase-carrying exosomes can cross a BMEC monolayer under stroke-like, inflamed conditions (TNF-α activated) but not under normal conditions. Confocal microscopy showed that exosomes are internalized by BMECs through endocytosis, co-localize with endosomes, in effect primarily utilizing the transcellular route of crossing. Together, these results indicate that cell-derived exosomes can cross the BBB model under stroke-like conditions in vitro. This study encourages further development of engineered exosomes as drug delivery vehicles or tracking tools for treating or monitoring neurological diseases.
- Subjects :
- 0301 basic medicine
Endosome
transcytosis
Biology
Endocytosis
Blood–brain barrier
Exosome
General Biochemistry, Genetics and Molecular Biology
Article
03 medical and health sciences
In vivo
medicine
exosome
endocytosis
blood-brain barrier (BBB)
humanized Gaussia luciferase (hGluc)
stroke
Microvesicles
3. Good health
Cell biology
030104 developmental biology
medicine.anatomical_structure
Transcytosis
inflammation
Modeling and Simulation
Immunology
Drug delivery
cardiovascular system
exocytosis
Subjects
Details
- Language :
- English
- ISSN :
- 18655033 and 18655025
- Volume :
- 9
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Cellular and molecular bioengineering
- Accession number :
- edsair.doi.dedup.....4a79693907e82c7d477ee8926a2d3485