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Molecular identification of panax ginseng c.a. meyer in ginseng commercial products
- Source :
- Scopus-Elsevier
-
Abstract
- Molecular techniques (PCR and RFLP) were used to verify the presence of Panax ginseng C.A. Meyer in commercial products containing ginseng. DNA, extracted from four vegetable forms present in marketed products, was amplified with 18df/28ccr primers. The RFLP of the DNA amplified products, obtained using Inf I, Sau 3A1 and Taq I endonucleases, allowed the identification of P. ginseng and its differentiation from P. quinquefolium. P. ginseng was detected in 9 out 16 samples tested which, according to the declaration on the labels, contained the drug. Negative results were obtained for products containing the dried extract of the drug. A comparison of the results acquired using the molecular techniques with those using HPLC is also reported.
- Subjects :
- Pharmacology
Traditional medicine
010405 organic chemistry
food and beverages
Plant Science
General Medicine
Biology
complex mixtures
01 natural sciences
0104 chemical sciences
010404 medicinal & biomolecular chemistry
Ginseng
Complementary and alternative medicine
Drug Discovery
Restriction fragment length polymorphism
Molecular identification
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Scopus-Elsevier
- Accession number :
- edsair.doi.dedup.....4a856bb242bca376ddda8b9b2155ef1a