Back to Search
Start Over
Multiplex qRT-PCR for the Detection of Western Equine Encephalomyelitis, St. Louis Encephalitis, and West Nile Viral RNA in Mosquito Pools (Diptera: Culicidae)
- Source :
- Journal of Medical Entomology. 52:491-499
- Publication Year :
- 2015
- Publisher :
- Oxford University Press (OUP), 2015.
-
Abstract
- Following the introduction of West Nile virus into California during the summer of 2003, public health and vector control programs expanded surveillance efforts and were in need of diagnostics capable of rapid, sensitive, and specific detection of arbovirus infections of mosquitoes to inform decision support for intervention. Development of a multiplex TaqMan or real-time semiquantitative reverse transcription polymerase chain reaction (RT-PCR) assay in which three virus specific primer-probe sets were used in the same reaction is described herein for the detection of western equine encephalomyelitis, St. Louis encephalitis and West Nile viral RNA. Laboratory validation and field data from 10 transmission seasons are reported. The comparative sensitivity and specificity of this multiplex assay to singleplex RT-PCR as well as an antigen detection (rapid analyte measurement platform) and standard plaque assays indicate this assay to be rapid and useful in providing mosquito infection data to estimate outbreak risk.
- Subjects :
- Vector-Borne Diseases, Surveillance, Prevention
Encephalitis Virus, St. Louis
Biology
medicine.disease_cause
Sensitivity and Specificity
California
Encephalitis Virus, Western Equine
Virus
Multiplex polymerase chain reaction
medicine
TaqMan
Animals
Multiplex
Western equine encephalitis virus
Encephalitis, St. Louis
General Veterinary
St louis encephalitis
Encephalomyelitis, Western Equine
medicine.disease
Virology
Reverse transcription polymerase chain reaction
Culicidae
Infectious Diseases
Insect Science
RNA, Viral
Parasitology
Multiplex Polymerase Chain Reaction
West Nile virus
West Nile Fever
Encephalitis
Subjects
Details
- ISSN :
- 19382928 and 00222585
- Volume :
- 52
- Database :
- OpenAIRE
- Journal :
- Journal of Medical Entomology
- Accession number :
- edsair.doi.dedup.....4a8740d2b3d5ed5671219eafafd82906
- Full Text :
- https://doi.org/10.1093/jme/tjv021