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Airway Smooth Muscle Cell Proliferation Is Increased in Asthma
- Source :
- American Journal of Respiratory and Critical Care Medicine. 164:474-477
- Publication Year :
- 2001
- Publisher :
- American Thoracic Society, 2001.
-
Abstract
- Increased airway smooth muscle (ASM) within the bronchial wall of asthmatic patients has been well documented and is likely to be the result of increased muscle proliferation. We have for the first time been able to culture ASM cells from asthmatic patients and to compare their proliferation rate with that of nonasthmatic patients. Asthmatic ASM cell cultures (n = 12) were established from explanted lungs and endobronchial biopsies. Nonasthmatic ASM cells (n = 10) were obtained from explanted tissue from patients with no airway disease, emphysema, carcinoma, and fibrosing alveolitis. Cell counts, tritiated thymidine incorporation, and cell cycle analysis were conducted over 7 d. Asthmatic ASM cell numbers at Days 3, 5, and 7 were significantly higher than corresponding values for nonasthmatic cells (p0.05). Tritiated thymidine incorporation was increased 3.2-fold in asthmatic cells compared with nonasthmatic cells within the first 24 h (p = 0.026). Flow cytometric analysis of DNA content on Days 1 and 2 revealed that a significantly greater percentage of asthmatic ASM cells were in the G2 + M phase (p0.05). This study shows for the first time that proliferation of ASM cells is increased in patients with asthma and provides evidence for an intrinsic abnormality in the ASM cell in this disease.asthma; human airway smooth muscle; cell culture; cell proliferation; hyperplasia
- Subjects :
- Adult
Male
Pulmonary and Respiratory Medicine
Pathology
medicine.medical_specialty
Adolescent
Respiratory System
Cell
Cell Culture Techniques
Critical Care and Intensive Care Medicine
Flow cytometry
medicine
Carcinoma
Humans
Respiratory system
Aged
medicine.diagnostic_test
business.industry
Respiratory disease
Muscle, Smooth
Middle Aged
respiratory system
Flow Cytometry
medicine.disease
Asthma
Pathophysiology
respiratory tract diseases
medicine.anatomical_structure
Cell culture
Female
business
Cell Division
Respiratory tract
Subjects
Details
- ISSN :
- 15354970 and 1073449X
- Volume :
- 164
- Database :
- OpenAIRE
- Journal :
- American Journal of Respiratory and Critical Care Medicine
- Accession number :
- edsair.doi.dedup.....4aa7491010f5ffe29c28e7331b9e3189