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Salivary N-glycosylation as a biomarker of oral cancer: A pilot study
- Source :
- Glycobiology. 29:726-734
- Publication Year :
- 2019
- Publisher :
- Oxford University Press (OUP), 2019.
-
Abstract
- Reliable biomarkers for oral cancer (OC) remain scarce, and routine tests for the detection of precancerous lesions are not routine in the clinical setting. This study addresses a current unmet need for more sensitive and quantitative tools for the management of OC. Whole saliva was used to identify and characterize the nature of glycans present in saliva and determine their potential as OC biomarkers. Proteins obtained from whole saliva were subjected to PNGase F enzymatic digestion. The resulting N-glycans were analyzed with weak anion exchange chromatography, exoglycosidase digestions coupled to ultra-high performance liquid chromatography and/or mass spectrometry. To determine N-glycan changes, 23 individuals with or without cancerous oral lesions were analyzed using Hydrophilic interaction ultra performance liquid chromatography (HILIC–UPLC), and peak-based area relative quantitation was performed. An abundant and complex salivary N-glycomic profile was identified. The main structures present in saliva were neutral oligosaccharides consisting of high mannose, hybrid and complex structures, followed by smaller fractions of mono and di-sialylated structures. To determine if differential N-glycosylation patterns distinguish between OC and control groups, Mann–Whitney testing and principle component analysis (PCA) were used. Eleven peaks were shown to be statistically significant (P ≤ 0.05), while PCA analysis showed segregation of the two groups based on their glycan profile. N-glycosylation changes are active in the oral carcinogenic process and may serve as biomarkers for early detection to reduce morbidity and mortality. Identifying which N-glycans contribute most in the carcinogenic process may lead to their use in the detection, prognosis and treatment of OC.
- Subjects :
- Adult
Male
PNGase F
Saliva
Glycan
Glycosylation
Glycoside Hydrolases
Oligosaccharides
Biochemistry
Mass Spectrometry
03 medical and health sciences
0302 clinical medicine
N-linked glycosylation
Polysaccharides
Exoglycosidase
Biomarkers, Tumor
medicine
Humans
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Chromatography, High Pressure Liquid
Early Detection of Cancer
Aged
030304 developmental biology
Aged, 80 and over
Principal Component Analysis
0303 health sciences
biology
Glycobiology
Chemistry
Cancer
Middle Aged
medicine.disease
030220 oncology & carcinogenesis
biology.protein
Biomarker (medicine)
Female
Mouth Neoplasms
Mannose
Subjects
Details
- ISSN :
- 14602423
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Glycobiology
- Accession number :
- edsair.doi.dedup.....4af7e7ae59c548c658eda2a05f33022d