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Repressible chloroplast gene expression in Chlamydomonas: A new tool for the study of the photosynthetic apparatus
- Source :
- Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1837(9), 1548-1552. Elsevier, Biochimica et Biophysica Acta (BBA)-Bioenergetics, Dinc, E, Ramundo, S, Croce, R & Rochaix, J D 2014, ' Repressible chloroplast gene expression in Chlamydomonas: A new tool for the study of the photosynthetic apparatus ', Biochimica et Biophysica Acta (BBA)-Bioenergetics, vol. 1837, no. 9, pp. 1548-1552 . https://doi.org/10.1016/j.bbabio.2013.11.020
- Publisher :
- Elsevier B.V.
-
Abstract
- A repressible/inducible chloroplast gene expression system has been used to conditionally inhibit chloroplast protein synthesis in the unicellular alga Chlamydomonas reinhardtii. This system allows one to follow the fate of photosystem II and photosystem I and their antennae upon cessation of chloroplast translation. The main results are that the levels of the PSI core proteins decrease at a slower rate than those of PSII. Amongst the light-harvesting complexes, the decrease of CP26 proceeds at the same rate as for the PSII core proteins whereas it is significantly slower for CP29, and for the antenna complexes of PSI this rate is comprised between that of CP26 and CP29. In marked contrast, the components of trimeric LHCII, the major PSII antenna, persist for several days upon inhibition of chloroplast translation. This system offers new possibilities for investigating the biosynthesis and turnover of individual photosynthetic complexes in the thylakoid membranes. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: Keys to Produce Clean Energy. © 2013 Elsevier B.V.
- Subjects :
- Photoinhibition
Photosystem II
Biophysics
Chlamydomonas reinhardtii
macromolecular substances
Photosystem I
Thylakoids
Biochemistry
Chloroplast
Stroma
Genes, Chloroplast
Botany
SDG 7 - Affordable and Clean Energy
Photosynthesis
Photosystem I Protein Complex
biology
Chlamydomonas
Photosystem II Protein Complex
food and beverages
Cell Biology
biology.organism_classification
Photosystem degradation
Thylakoid
Gene expression
Subjects
Details
- Language :
- English
- ISSN :
- 00052728
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - Bioenergetics
- Accession number :
- edsair.doi.dedup.....4afbac2cbbfba599f2575212226f020f
- Full Text :
- https://doi.org/10.1016/j.bbabio.2013.11.020