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Lung transcriptome of nonhuman primates exposed to total- and partial-body irradiation
- Source :
- Molecular therapy. Nucleic acids. 29
- Publication Year :
- 2022
-
Abstract
- The focus of radiation biodosimetry has changed recently, and a paradigm shift for using molecular technologies of omic platforms in addition to cytogenetic techniques has been observed. In our study, we have used a nonhuman primate model to investigate the impact of a supralethal dose of 12 Gy radiation on alterations in the lung transcriptome. We used 6 healthy and 32 irradiated animal samples to delineate radiation-induced changes. We also used a medical countermeasure, γ-tocotrienol (GT3), to observe any changes. We demonstrate significant radiation-induced changes in the lung transcriptome for total-body irradiation (TBI) and partial-body irradiation (PBI). However, no major influence of GT3 on radiation was noted in either comparison. Several common signaling pathways, including PI3K/AKT, GADD45, and p53, were upregulated in both exposures. TBI activated DNA-damage-related pathways in the lungs, whereas PTEN signaling was activated after PBI. Our study highlights the various transcriptional profiles associated with γ- and X-ray exposures, and the associated pathways include LXR/RXR activation in TBI, whereas pulmonary wound-healing and pulmonary fibrosis signaling was repressed in PBI. Our study provides important insights into the molecular pathways associated with irradiation that can be further investigated for biomarker discovery.
- Subjects :
- Drug Discovery
Molecular Medicine
Subjects
Details
- ISSN :
- 21622531
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Molecular therapy. Nucleic acids
- Accession number :
- edsair.doi.dedup.....4b049cb9219f660e64c5eaf70d6db4fa