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In vivo application and dynamics of lactic acid bacteria for the four-season production of Vastedda-like cheese
- Publication Year :
- 2014
- Publisher :
- ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS, 2014.
-
Abstract
- article Twelve lactic acid bacteria (LAB), previously selected in vitro (Gaglio et al., 2014), were evaluated in situ for their potential to act as starter cultures for the continuous four-season production of Vastedda-like cheese, made with raw ewes' milk. The strains belonged to Lactobacillus delbrueckii, Lactococcus lactis subsp. cremoris, Leuconostoc mesenteroides subsp. mesenteroides and Streptococcus thermophilus .L AB werefirst inoculated in multiple-strain combinations on the basis of their optimal growth temperatures in three process conditions which differed for milk treatment and medium for strain development: process 1, growth of strains in the optimal synthetic media and pasteurised milk; process 2, growth of strains in whey based medium (WBM) and pasteurised milk; and process 3, growth of strains in WBM and raw milk. The strains that acidified the curds in short time, as shown by a pH drop, were all mesophilic and were then tested in a single inoculum through process 3. Ran- domly amplified polymorphic DNA (RAPD)-PCR analysis applied to the colonies isolated from the highest dilu- tions of samples confirmed the dominance of the added strains after curd acidification, stretching and storage. After 15 days of refrigerated storage, the decrease in pH values showed an activity of the mesophilic strains at low temperatures, but only Lc. lactis subsp. cremoris PON153, Ln. mesenteroides subsp. mesenteroides PON259 and PON559 increased their number during the 15 days at 7 °C. A sensory evaluation indicated that the cheeses obtained by applying protocol 3 and by inoculation with lactococci are the most similar to the protected denom- ination oforigin(PDO) cheese andreceivedthebest scores bythe judges. Thus,theexperimentalcheesesobtain- ed with raw milk and inoculated with single and multiple combinations of lactococci were subjected to the analysis of the volatile organic compounds (VOCs) carried out by a headspace solid phase microextraction (SPME) technique coupled with gas chromatography with mass spectrometric detection (GC/MS). The domi- nance of lactococci over thermophilic LAB of raw milk was verified during summer production and, based on the combination of VOC profiles and sensory evaluation of the final cheeses, the multi-strain Lactococcus culture resulted in the most suitable starter preparation for the full-year production of Vastedda-like cheese.
- Subjects :
- Streptococcus thermophilus
Lactococcus
Gram-Positive Bacteria
Microbiology
Fermentation
Lactic acid bacteria
Pilot plant
Raw milk
Starter cultures
Traditional cheese
Settore AGR/17 - Zootecnica Generale E Miglioramento Genetico
Cheese
Lactobacillus
Leuconostoc
Food microbiology
Animals
Humans
Food science
Lactobacillus delbrueckii
Volatile Organic Compounds
biology
Lactococcus lactis
Temperature
food and beverages
General Medicine
Settore AGR/15 - Scienze E Tecnologie Alimentari
Hydrogen-Ion Concentration
biology.organism_classification
Random Amplified Polymorphic DNA Technique
Milk
Starter culture
Leuconostoc mesenteroides
Taste
Food Microbiology
Seasons
Food Science
Settore AGR/16 - Microbiologia Agraria
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....4b1d0f30a1990288ed228a2916d9b4db