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A unique bacterial secretion machinery with multiple secretion centers

Authors :
Liqiang Song
John D. Perpich
Chenggang Wu
Thierry Doan
Zuzanna Nowakowska
Jan Potempa
Peter J. Christie
Eric Cascales
Richard J. Lamont
Bo Hu
Department of Microbiology and Molecular Genetics
The University of Texas Health Science Center at Houston (UTHealth)
University of Louisville School of Medicine
Department of Microbiology & Molecular Genetics
Laboratoire d'ingénierie des systèmes macromoléculaires (LISM)
Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Uniwersytet Jagielloński w Krakowie = Jagiellonian University (UJ)
ANR-20-CE11-0011,T9-Mechanism,Elucider le mécanisme d'action du système de sécrétion de type IX (T9SS)(2020)
Source :
Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences of the United States of America, 2022, 119 (18), ⟨10.1073/pnas.2119907119⟩
Publication Year :
2022

Abstract

Significance The newly described type IX secretion systems (T9SSs) translocate virulence factors and can mediate specialized gliding motility among bacterial pathogens of the Fibrobacteres–Chlorobi–Bacterioidetes superphylum. We visualized the spatial organization of the T9SS in its native context in the Porphorymonas gingivalis cell by cryoelectron tomography. The T9SS exhibits distinct symmetries across the bacterial cell envelope: a cytoplasmic complex requiring PorL and PorM for assembly exhibits 12-fold symmetry; a periplasmic complex composed of PorM exhibits 18-fold symmetry and attaches to a PorKN ring near the outer membrane; and eight Sov translocons are arranged with 8-fold symmetry at the cell surface. The T9SS is the largest of the known bacterial secretion systems and evidently arranges as multiple, independently functioning translocation motors.

Details

ISSN :
10916490 and 00278424
Volume :
119
Issue :
18
Database :
OpenAIRE
Journal :
Proceedings of the National Academy of Sciences of the United States of America
Accession number :
edsair.doi.dedup.....4b551308b756e2fa3b689d16496f3317
Full Text :
https://doi.org/10.1073/pnas.2119907119⟩