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Critical roles of adenosine A2A receptor in regulating the balance of Treg/Th17 cells in allergic asthma
- Source :
- The clinical respiratory journal. 12(1)
- Publication Year :
- 2015
-
Abstract
- Introduction Deficiency of Treg cells and hyperactivity of Th17 cells together are involved in the immunological pathogenesis of asthma. The adenosine A2A receptor (A2AR) plays a critical role in the increased Foxp3 expression of Treg cells and the decreased Th17 generation. Objective The study aimed to investigate A2AR expression in peripheral blood and its regulatory effect on balance of Treg/Th17 cells in asthma. Methods Thirty-one patients with chronic persistent asthma were recruited and divided into 18 intermittent to mild asthma patients, 13 moderate to severe asthma patients. A2AR, Foxp3, and ROR-γt mRNA expression levels in peripheral blood mononuclear cells (PBMCs) were measured by quantitative polymerase chain reaction (qPCR). TGF-β, IL-17, and IgE in plasma were detected with enzyme-linked immunosorbent assay (ELISA). Forty-two BALB/c mice were randomly, equally assigned to control group, ovalbumin (OVA) group and OVA + CGS (CGS21680, A2AR agonist) group. The infiltration of lung inflammation cells were evaluated by HE, A2AR, Foxp3, and ROR-γt mRNA in lung tissues measured by qPCR, TGF-β, IL-17, and IgE in plasma measured with ELISA, and IL-17 and TGF-β protein in lung tissues analyzed with immunohistochemical. Results Our results showed that expression A2AR mRNA in PBMCs was associated with asthma severity. Foxp3 mRNA, TGF-β, and FEV1%pred positively correlated with A2AR mRNA in asthma. ROR-γt mRNA and IL-17 negatively correlated with A2AR mRNA in asthma. CGS could promote Foxp3 mRNA expression, TGF-β, and improve lung function while inhibit ROR-γt mRNA expression, IL-17, and the infiltration of lung inflammation cells. Conclusion A2AR could regulate the balance of Treg/Th17 cells in asthma.
- Subjects :
- 0301 basic medicine
Pulmonary and Respiratory Medicine
Adult
Male
medicine.medical_specialty
Receptor, Adenosine A2A
Inflammation
Enzyme-Linked Immunosorbent Assay
Immunoglobulin E
Peripheral blood mononuclear cell
Polymerase Chain Reaction
T-Lymphocytes, Regulatory
03 medical and health sciences
0302 clinical medicine
Internal medicine
Immunology and Allergy
Medicine
Animals
Humans
RNA, Messenger
Genetics (clinical)
Asthma
Mice, Inbred BALB C
Lung
biology
business.industry
FOXP3
hemic and immune systems
medicine.disease
Immunohistochemistry
respiratory tract diseases
Ovalbumin
Disease Models, Animal
030104 developmental biology
Real-time polymerase chain reaction
Endocrinology
medicine.anatomical_structure
Gene Expression Regulation
Immunology
biology.protein
Leukocytes, Mononuclear
Cytokines
Th17 Cells
Female
medicine.symptom
business
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 1752699X
- Volume :
- 12
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- The clinical respiratory journal
- Accession number :
- edsair.doi.dedup.....4b7352008aadfe6c6f47aab69b82c9c9