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N-linked glycopeptides with blood group determinants lacking neuraminic acid from the epithelial cells of rat small and large intestine
- Source :
- European journal of biochemistry. 203(1-2)
- Publication Year :
- 1992
-
Abstract
- The N-linked type of glycans were prepared as their glycopeptides after pronase digestion of the epithelial cells from the small and large intestine of two inbred strains of rat. These glycopeptides were analysed for sugar composition, for blood-group activity, by 1H-NMR spectroscopy, and after permethylation by electron-impact mass spectrometry. The glycopeptides were of the triantennary and tetraantennary types with intersected GlcNAc. The terminal parts were, in contrast to most N-linked glycans, devoid of neuraminic acid residues. Instead they contained blood-group determinants. Blood-group-H types 1 (Fuc alpha 1-2Gal beta 1-3GlcNAc) and 2(Fuc alpha 1-2Gal beta 1-4GlcNAc) were found in the small and large intestines of both strains, although type-1 predominated. One rat strain (GOT-W) did not express blood-group-A glycopeptides in the small intestine, but the large intestine from the same strain did. The other strain (GOT-BW) expressed blood-group-A determinants in the small intestine. The lack of neuraminic acid residues in the small and large intestine and of blood-group-B activity in the large intestine differed from that found in glycosphingolipids obtained from the same organs.
- Subjects :
- Glycan
Magnetic Resonance Spectroscopy
Biology
Biochemistry
Glycosphingolipids
Mass Spectrometry
chemistry.chemical_compound
Inbred strain
Neuraminic acid
Intestine, Small
medicine
Animals
Humans
Large intestine
Intestine, Large
Intestinal Mucosa
Strain (chemistry)
Glycopeptides
Epithelium
Glycopeptide
Small intestine
Rats
medicine.anatomical_structure
chemistry
biology.protein
Blood Group Antigens
Chromatography, Gel
Neuraminic Acids
Subjects
Details
- ISSN :
- 00142956
- Volume :
- 203
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- European journal of biochemistry
- Accession number :
- edsair.doi.dedup.....4b74d4faf0ea87168a25bcf7a2d9611a