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Evaluation of Carum-loaded Niosomes on Breast Cancer Cells:Physicochemical Properties, In Vitro Cytotoxicity, Flow Cytometric, DNA Fragmentation and Cell Migration Assay
Evaluation of Carum-loaded Niosomes on Breast Cancer Cells:Physicochemical Properties, In Vitro Cytotoxicity, Flow Cytometric, DNA Fragmentation and Cell Migration Assay
- Source :
- Scientific Reports, Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
- Publication Year :
- 2018
-
Abstract
- Thymoquinone (TQ), a phytochemical compound found in Carum carvil seeds (C. carvil), has a lot of applications in medical especially cancer therapy. However, TQ has a hydrophobic nature, and because of that, its solubility, permeability and its bioavailability in biological mediums are poor. To diminish these drawbacks, we have designed a herbal carrier composed of Ergosterol (herbal lipid), Carum carvil extract (Carum) and nonionic surfactants for herbal cancer treatment. C. carvil was extracted and characterized by GC/Mass. Two different formulations containing TQ and Carum were encapsulated into niosomes (Nio/TQ and Nio/Carum, respectively) and their properties were compared together. Morphology, size, zeta potential, encapsulation efficiency (EE%), profile release rate, in vitro cytotoxicity, flow cytometric, DNA fragmentation and cell migration assay of formulations were evaluated. Results show that both loaded formulations have a spherical morphology, nanometric size and negative zeta potential. EE% of TQ and Carum loaded niosomes was about 92.32% ± 2.32 and 86.25% ± 1.85, respectively. Both loaded formulations provided a controlled release compared with free TQ. MTT assay showed that loaded niosomes have more anti-cancer activity compared with Free TQ and free Carum against MCF-7 cancer cell line and these results were confirmed by flow cytometric analysis. Cell cycle analysis showed G2/M arrest in TQ, Nio/TQ and Nio/Carum formulations. TQ, Nio/TQ and Nio/Carum decreased the migration of MCF7 cells remarkedly. These results show that the TQ and Carum loaded niosomes are novel carriers with high efficiency for encapsulation of low soluble phytochemicals and also would be favourable systems for breast cancer treatment.
- Subjects :
- 0301 basic medicine
Cell Survival
lcsh:Medicine
Breast Neoplasms
DNA Fragmentation
Carum
Article
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Breast cancer
Cell Movement
Zeta potential
Benzoquinones
Electron microscopy
Humans
MTT assay
Niosome
lcsh:Science
Thymoquinone
Cell Proliferation
Multidisciplinary
Chromatography
biology
Chemistry
Plant Extracts
lcsh:R
biology.organism_classification
Controlled release
Antineoplastic Agents, Phytogenic
Bioavailability
030104 developmental biology
Phytochemical
Delayed-Action Preparations
Liposomes
MCF-7 Cells
lcsh:Q
Female
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....4b7e5658e31fb32d0cec0a99f9ecf02e