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Long noncoding RNA NEAT1 promotes laryngeal squamous cell cancer through regulating miR-107/CDK6 pathway
- Source :
- Journal of Experimental & Clinical Cancer Research : CR
- Publisher :
- Springer Nature
-
Abstract
- Background Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) plays key role in the progression of some human cancers. However, the role of NEAT1 in human laryngeal squamous cell cancer (LSCC) is still unknown. We therefore investigated the expression and function of NEAT1 in LSCC. Methods NEAT1 level in LSCC and adjacent non-neoplastic tissues were detected by qRT-PCR. NEAT1 was knockdown in LSCC cells and cell proliferation, apoptosis and cell cycle were examined. The growth of xenografts with NEAT1 knockdown LSCC cells was analyzed. Results NEAT1 level was significantly higher in LSCC than in corresponding adjacent non-neoplastic tissues, and patients with neck nodal metastasis or advanced clinical stage had higher NEAT1 expression. Moreover, siRNA mediated NEAT1 knockdown significantly inhibited the proliferation and induced apoptosis and cell cycle arrest at G1 phase in LSCC cells. The growth of LSCC xenografts was significantly suppressed by the injection of NEAT1 siRNA lentivirus. Furthermore, NEAT1 regulated CDK6 expression in LSCC cells which was mediated by miR-107. Conclusion NEAT1 plays an oncogenic role in the tumorigenesis of LSCC and may serve as a potential target for therapeutic intervention.
- Subjects :
- 0301 basic medicine
Oncology
Cancer Research
medicine.medical_specialty
Cell cycle checkpoint
CDK6
NEAT1
Laryngeal squamous cell cancer
Biology
medicine.disease_cause
03 medical and health sciences
Mice
0302 clinical medicine
Internal medicine
Cell Line, Tumor
microRNA
medicine
Animals
Humans
Laryngeal Neoplasms
Gene knockdown
Cell growth
Research
miR-107
Cell Cycle
Cyclin-Dependent Kinase 6
Cell cycle
Gene Expression Regulation, Neoplastic
MicroRNAs
030104 developmental biology
Apoptosis
030220 oncology & carcinogenesis
Cancer research
biology.protein
Carcinoma, Squamous Cell
RNA, Long Noncoding
Cyclin-dependent kinase 6
Carcinogenesis
Neoplasm Transplantation
Long noncoding RNA
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 17569966
- Volume :
- 35
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental & Clinical Cancer Research
- Accession number :
- edsair.doi.dedup.....4b8bcbb0159b3e622787699caa87474c
- Full Text :
- https://doi.org/10.1186/s13046-016-0297-z