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Odontoblastic inductive potential of epithelial cells derived from human deciduous dental pulp
- Source :
- Journal of Molecular Histology. 47:345-351
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- For the dentin regeneration, dental epithelial cells are indispensible and must possess odontoblastic induction capability. Epithelial cell-like stem cells were recently identified in human deciduous dental pulp (DPESCs). However, their cellular characteristics remain poorly defined. The purpose of this study was to characterize DPESCs compared to HAT-7 ameloblastic cells. Expression levels of ameloblast-specific markers [odontogenic ameloblast-associated protein (Odam), matrix metalloproteinase (Mmp)-20, amelogenin, and ameloblastin] were detected in DPESCs. Co-culturing odontoblastic MDPC-23 cells with DPESCs increased expression of odontoblast differentiation markers (Dmp1 and Dspp) from days 4 to 10, while the expression of bone sialoprotein rapidly decreased. MDPC-23 cells cultured in DPESC-conditioned medium (CM) showed increased Dspp promoter activity compared with control MDPC-23 cultures. Mineralization was first observed in the CM groups from day 4 and proceeded rapidly until day 14, whereas mineralized nodules were found from day 7 in control media-cultured cells. In conclusion, DPESCs in human deciduous pulp possess ameloblast-like characteristics and differentiation properties, and substances derived from DPESCs promote odontoblastic differentiation. Thus, our results indicate that DPESCs can be a realistic epithelial source for use in odontoblastic induction and dentin formation of dental mesenchymal cells.
- Subjects :
- Transcriptional Activation
0301 basic medicine
Bone sialoprotein
Pathology
medicine.medical_specialty
Histology
Physiology
Cellular differentiation
Gene Expression
Odontoblast differentiation
03 medical and health sciences
0302 clinical medicine
stomatognathic system
medicine
Humans
Promoter Regions, Genetic
Cells, Cultured
Dental Pulp
Odontoblasts
biology
Chemistry
Mesenchymal stem cell
Cell Differentiation
Epithelial Cells
Cell Biology
General Medicine
DMP1
Cell biology
stomatognathic diseases
030104 developmental biology
Odontoblast
030220 oncology & carcinogenesis
biology.protein
Pulp (tooth)
Stem cell
Biomarkers
Subjects
Details
- ISSN :
- 15672387 and 15672379
- Volume :
- 47
- Database :
- OpenAIRE
- Journal :
- Journal of Molecular Histology
- Accession number :
- edsair.doi.dedup.....4bb6398a7e0955522be918fbc1d849fa