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Recent advances in the synthesis of rare sugars using DHAP-dependent aldolases
- Source :
- Carbohydrate research. 452
- Publication Year :
- 2017
-
Abstract
- The occurrence rates of non-communicable diseases like obesity, diabetes and hyperlipidemia have increased remarkably due to excessive consumption of a high-energy diet. Rare sugars therefore have become increasingly attractive owing to their unique nutritional properties. In the past two decades, various rare sugars have been successfully prepared guided by the “Izumoring strategy”. As a valuable complement to the Izumoring approach, the controllable dihydroxyacetone phosphate (DHAP)-dependent aldolases have generally predictable regio- and stereoselectivity, which makes them powerful tools in C-C bond construction and rare sugar production. However, the main disadvantage for this group of aldolases is their strict substrate specificity toward the donor molecule DHAP, a very expensive and relatively unstable compound. Among the current methods involving DHAP, the one that couples DHAP production from inexpensive starting materials (for instance, glycerol, DL-glycerol 3-phosphate, dihydroxyacetone, and glucose) with aldol condensation appears to be the most promising. This review thus focuses on recent advances in the application of L-rhamnulose-1-phosphate aldolase (RhaD), L-fuculose-1-phosphate aldolase (FucA), and D-fructose-1,6-bisphosphate aldolase (FruA) for rare sugar synthesis in vitro and in vivo, while illustrating strategies for supplying DHAP in efficient and economical ways.
- Subjects :
- biology
010405 organic chemistry
Organic Chemistry
Aldolase A
Dihydroxyacetone
General Medicine
Enzymatic synthesis
010402 general chemistry
Rare sugar
01 natural sciences
Biochemistry
0104 chemical sciences
Analytical Chemistry
Substrate Specificity
chemistry.chemical_compound
chemistry
Dihydroxyacetone Phosphate
DHAP
biology.protein
Substrate specificity
Aldol condensation
Sugars
Dihydroxyacetone phosphate
Aldehyde-Lyases
Subjects
Details
- ISSN :
- 1873426X
- Volume :
- 452
- Database :
- OpenAIRE
- Journal :
- Carbohydrate research
- Accession number :
- edsair.doi.dedup.....4bcefe75cb3a3afc79ca7cf4ddf27f56