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Optimizing illumination in three-dimensional deconvolution microscopy for accurate refractive index tomography
- Source :
- Optics express. 29(5)
- Publication Year :
- 2021
-
Abstract
- In light transmission microscopy, axial scanning does not directly provide tomographic reconstruction of specimen. Phase deconvolution microscopy can convert a raw intensity image stack into a refractive index tomogram, the intrinsic sample contrast which can be exploited for quantitative morphological analysis. However, this technique is limited by reconstruction artifacts due to unoptimized optical conditions, which leads to a sparse and non-uniform optical transfer function. Here, we propose an optimization method based on simulated annealing to systematically obtain optimal illumination schemes that enable artifact-free deconvolution. The proposed method showed precise tomographic reconstruction of unlabeled biological samples.
- Subjects :
- Materials science
Light
02 engineering and technology
01 natural sciences
010309 optics
Optics
Imaging, Three-Dimensional
Optical transfer function
0103 physical sciences
Microscopy
Humans
Tomography, Optical
Microscopy, Phase-Contrast
Colloids
Tomographic reconstruction
business.industry
021001 nanoscience & nanotechnology
Dark field microscopy
eye diseases
Atomic and Molecular Physics, and Optics
Interference microscopy
Microspheres
Refractometry
HEK293 Cells
sense organs
Tomography
Deconvolution
0210 nano-technology
business
Refractive index
Algorithms
Subjects
Details
- ISSN :
- 10944087
- Volume :
- 29
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Optics express
- Accession number :
- edsair.doi.dedup.....4bfe4280aed9b4249f7799fde17fc2f3