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Cloning and characterization of the gene for L-amino acid oxidase in hybrid tilapia
- Source :
- Molecular biology reports. 42(12)
- Publication Year :
- 2015
-
Abstract
- Tilapia is the common name for a group of cichlid fishes. Identification of DNA markers significantly associated with important traits in candidate genes may speed up genetic improvement. L-Amino acid oxidase (LAO) plays a crucial role in the innate immune defences of animals. Previously, whether LAO variants were associated with economic traits had not been studied in fish. We characterized the cDNA sequence of the LAO gene of hybrid tilapia (Oreochromis spp.). Its ORF was 1536 bp, encoding a flavoenzyme of 511 amino acids. This gene consisted of seven exons and six introns. Its expression was detected in the intestine, blood, kidney, skin, liver. It was highly expressed in the intestine. After a challenge with a bacterial pathogen, Streptococcus agalactiae, its expression was up-regulated significantly in the liver, intestine and spleen (P
- Subjects :
- Fish Proteins
Male
Candidate gene
food.ingredient
Molecular Sequence Data
Biology
L-Amino Acid Oxidase
Streptococcus agalactiae
Exon
food
Complementary DNA
Streptococcal Infections
Genetics
Animals
Amino Acid Sequence
Cloning, Molecular
Molecular Biology
Gene
Phylogeny
Disease Resistance
Regulation of gene expression
Tilapia
General Medicine
biology.organism_classification
Molecular biology
Oreochromis
Gene Expression Regulation
Genetic marker
Organ Specificity
Female
human activities
Sequence Alignment
Subjects
Details
- ISSN :
- 15734978
- Volume :
- 42
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Molecular biology reports
- Accession number :
- edsair.doi.dedup.....4c0aeca01428533d5699780607712be9