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Symbiotic and Asymbiotic Germination of Dendrobium officinale (Orchidaceae) Respond Differently to Exogenous Gibberellins
- Source :
- International Journal of Molecular Sciences, Volume 21, Issue 17
- Publication Year :
- 2020
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2020.
-
Abstract
- Seeds of almost all orchids depend on mycorrhizal fungi to induce their germination in the wild. The regulation of this symbiotic germination of orchid seeds involves complex crosstalk interactions between mycorrhizal establishment and the germination process. The aim of this study was to investigate the effect of gibberellins (GAs) on the symbiotic germination of Dendrobium officinale seeds and its functioning in the mutualistic interaction between orchid species and their mycobionts. To do this, we used liquid chromatograph-mass spectrometer to quantify endogenous hormones across different development stages between symbiotic and asymbiotic germination of D. officinale, as well as real-time quantitative PCR to investigate gene expression levels during seed germination under the different treatment concentrations of exogenous gibberellic acids (GA3). Our results showed that the level of endogenous GA3 was not significantly different between the asymbiotic and symbiotic germination groups, but the ratio of GA3 and abscisic acids (ABA) was significantly higher during symbiotic germination than asymbiotic germination. Exogenous GA3 treatment showed that a high concentration of GA3 could inhibit fungal colonization in the embryo cell and decrease the seed germination rate, but did not significantly affect asymbiotic germination or the growth of the free-living fungal mycelium. The expression of genes involved in the common symbiotic pathway (e.g., calcium-binding protein and calcium-dependent protein kinase) responded to the changed concentrations of exogenous GA3. Taken together, our results demonstrate that GA3 is probably a key signal molecule for crosstalk between the seed germination pathway and mycorrhiza symbiosis during the orchid seed symbiotic germination.
- Subjects :
- 0106 biological sciences
0301 basic medicine
symbiosis germination
Germination
orchid mycorrhiza
plant hormone
01 natural sciences
Article
Catalysis
Mass Spectrometry
Inorganic Chemistry
03 medical and health sciences
Orchid mycorrhiza
Symbiosis
Gene Expression Regulation, Plant
Mycorrhizae
Botany
Gene expression
Physical and Theoretical Chemistry
Mycorrhiza
Orchidaceae
Molecular Biology
Spectroscopy
Plant Proteins
biology
Sequence Analysis, RNA
Basidiomycota
Gene Expression Profiling
Organic Chemistry
General Medicine
biology.organism_classification
Gibberellins
Computer Science Applications
030104 developmental biology
Seeds
gene expression
Gibberellin
Plant hormone
010606 plant biology & botany
Abscisic Acid
Chromatography, Liquid
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....4c12501db2fffe7e312fc16066977d6e
- Full Text :
- https://doi.org/10.3390/ijms21176104