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Effects of NYGGF4 knockdown on insulin sensitivity and mitochondrial function in 3T3-L1 adipocytes

Authors :
Jia-Zheng Dai
Xirong Guo
Yan Cui
Xiling Li
Chen-Bo Ji
Chun-Mei Zhang
Min Zhang
Xue-Qi Zeng
Xia Chi
Rong Zhang
Meiling Tong
Source :
Journal of bioenergetics and biomembranes. 42(5)
Publication Year :
2010

Abstract

NYGGF4 is a recently discovered gene that is involved in obesity-associated insulin resistance. It has been suggested that mitochondrial dysfunction might be responsible for the development of insulin resistance induced by NYGGF4 overexpression. In the present study, we aimed to define the impact of down-regulating NYGGF4 expression by RNA interference (RNAi) on the insulin sensitivity and mitochondrial function of 3T3-L1 adipocytes. The results revealed that NYGGF4 knockdown enhanced the glucose uptake of adipocytes, which reconfirmed the regulatory function of NYGGF4 in adipocyte insulin sensitivity. However, an unexpected observation was that knockdown of NYGGF4 reduced intracellular ATP concentration and promoted an increase in mitochondrial transmembrane potential (ΔΨm) and reactive oxygen species (ROS) level without affecting mitochondrial morphology or mtDNA. Therefore, the role of NYGGF4 in mitochondrial function remains unclear, and further animal studies are needed to explore the biological function of this gene.

Details

ISSN :
15736881
Volume :
42
Issue :
5
Database :
OpenAIRE
Journal :
Journal of bioenergetics and biomembranes
Accession number :
edsair.doi.dedup.....4c197392a34496c09263d43e7a75179c