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Effect of β-escin sodium on endothelial cells proliferation, migration and apoptosis
- Source :
- Vascular Pharmacology. 49:158-165
- Publication Year :
- 2008
- Publisher :
- Elsevier BV, 2008.
-
Abstract
- beta-Escin, the major active compound in extracts of the horse chestnut Aesculus hippocastanum seed, has shown clinically significant activity in chronic venous insufficiency (CVI). Our previous studies had shown that beta-escin sodium inhibited angiogenesis in chick chorioallantoic membrane (CAM) and in aortic disk assay. In this study, we explored the direct effect of beta-escin sodium on proliferation, migration and apoptosis in human umbilical vein endothelial cells (HUVECs) and ECV304 cells. Sulforhodamine B (SRB) assay showed that beta-escin sodium (10, 20, 40 microg/ml) inhibited endothelial cells (ECs) proliferation dose-dependently. beta-escin sodium also induced ECs apoptosis at 40 microg/ml. Cell migration was evaluated by an improved wound assay: barren spot assay. And the direct effect on cell motility excluding influence of cell proliferation was examined by High Content Screening (HCS, Cellomics) assay. The data indicated that beta-escin sodium suppressed ECs migration and cell motility. Western blot results suggested that beta-escin sodium acts on ECs possibly by increasing expression of thrombospondin-1 (TSP-1), and decreasing expression of PKC-alpha and activation of p44/42 mitogen-activated protein kinase (ERK) and p38 mitogen-activated protein kinase (p38 MAPK). Our findings give the evidence that beta-escin sodium might have potential anti-angiogenic activity via its direct effects on ECs.
- Subjects :
- Protein Kinase C-alpha
Physiology
Angiogenesis
Sodium
Blotting, Western
Motility
chemistry.chemical_element
Apoptosis
Biology
p38 Mitogen-Activated Protein Kinases
Umbilical vein
Cell Line
Thrombospondin 1
Cell Movement
Humans
Phosphorylation
Extracellular Signal-Regulated MAP Kinases
Protein kinase A
Cells, Cultured
Cell Proliferation
Pharmacology
Analysis of Variance
Escin
Dose-Response Relationship, Drug
Cell growth
Endothelial Cells
Cell migration
Saponins
Flow Cytometry
Molecular biology
Biochemistry
chemistry
Molecular Medicine
Subjects
Details
- ISSN :
- 15371891
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- Vascular Pharmacology
- Accession number :
- edsair.doi.dedup.....4c231ceac20828bcff4ac56e8b94a3b8