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Compromised Production of Extracellular Matrix in Mice Lacking Secreted Protein, Acidic and Rich in Cysteine (SPARC) Leads to a Reduced Foreign Body Reaction to Implanted Biomaterials
- Source :
- The American Journal of Pathology. 162:627-635
- Publication Year :
- 2003
- Publisher :
- Elsevier BV, 2003.
-
Abstract
- SPARC (secreted protein, acidic and rich in cysteine), a matricellular glycoprotein, modulates the interaction of cells with the extracellular matrix (ECM). Recently, accelerated cutaneous wound closure and altered deposition of collagen were reported in SPARC-null mice. Herein we asked whether SPARC might influence the foreign body reaction to biomaterial implants. Polydimethylsiloxane (silicone rubber) disks and cellulose Millipore filters were implanted into wild-type and SPARC-null mice. In wild-type animals, significant levels of SPARC were observed in the cells and the ECM comprising the capsules around the implants. After 4 weeks, SPARC-null mice exhibited a significant decrease in the thickness of the foreign body capsule, as compared to that observed in wild-type mice. A significant reduction in capsular vascular density was also associated with the silicone implants in the SPARC-null animals. Electron microscopy revealed that collagen fibers in the capsules produced by SPARC-null mice were smaller and more uniform in size than those in wild-type animals. Furthermore, staining with picrosirius-red showed that the collagen fibers were less mature in SPARC-null than in wild-type mice. The altered ECM resulting in decreased capsular thickness, indicative of an altered foreign body reaction in SPARC-null mice, implicates SPARC as an important modulator of the encapsulation of implanted biomaterials.
- Subjects :
- Pathology
medicine.medical_specialty
Silicones
Biology
Pathology and Forensic Medicine
Extracellular matrix
Mice
chemistry.chemical_compound
Silicone
Coated Materials, Biocompatible
medicine
Animals
Osteonectin
Dimethylpolysiloxanes
Cells, Cultured
Mice, Knockout
chemistry.chemical_classification
Foreign-Body Reaction
Biomaterial
Prostheses and Implants
Fibroblasts
medicine.disease
Immunohistochemistry
Cell biology
Microscopy, Electron
Secretory protein
Bromodeoxyuridine
chemistry
biology.protein
Implant
Glycoprotein
Regular Articles
Foreign body granuloma
Subjects
Details
- ISSN :
- 00029440
- Volume :
- 162
- Database :
- OpenAIRE
- Journal :
- The American Journal of Pathology
- Accession number :
- edsair.doi.dedup.....4c30ceecdb430f7be18d20698d2b5f53