Back to Search Start Over

Visceral sensitivity modulation by faecal microbiota transplantation: The active role of gut bacteria in pain persistence

Authors :
Claudio Nicoletti
Siobhain M. O'Mahony
Alessandra Pacini
Gianluca Bartolucci
Lorenzo Di Cesare Mannelli
John F. Cryan
Alessandra Toti
Elena Niccolai
Gian Maria Rossolini
Laura Micheli
Vincenzo Di Pilato
Elena Lucarini
Carmen Parisio
Carla Ghelardini
Simone Baldi
Amedeo Amedei
Publication Year :
2021
Publisher :
Lippincott Williams and Wilkins, 2021.

Abstract

Recent findings linked gastrointestinal disorders characterized by abdominal pain to gut microbiota composition. The present work aimed to evaluate the power of gut microbiota as a visceral pain modulator and, consequently, the relevance of its manipulation as a therapeutic option in reversing postinflammatory visceral pain persistence. Colitis was induced in mice by intrarectally injecting 2,4-dinitrobenzenesulfonic acid (DNBS). The effect of faecal microbiota transplantation from viscerally hypersensitive DNBS-treated and naive donors was evaluated in control rats after an antibiotic-mediated microbiota depletion. Faecal microbiota transplantation from DNBS donors induced a long-lasting visceral hypersensitivity in control rats. Pain threshold trend correlated with major modifications in the composition of gut microbiota and short chain fatty acids. By contrast, no significant alterations of colon histology, permeability, and monoamines levels were detected. Finally, by manipulating the gut microbiota of DNBS-treated animals, a counteraction of persistent visceral pain was achieved. The present results provide novel insights into the relationship between intestinal microbiota and visceral hypersensitivity, highlighting the therapeutic potential of microbiota-targeted interventions.

Details

Language :
English
Database :
OpenAIRE
Accession number :
edsair.doi.dedup.....4c35e0c8eb57b59a1d787872db53e6f1