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Evaluation of the Genotypic Prediction of HIV-1 Coreceptor Use versus a Phenotypic Assay and Correlation with the Virological Response to Maraviroc: the ANRS GenoTropism Study
- Source :
- Antimicrobial Agents and Chemotherapy, Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 2010, 54 (8), pp.3335-40. ⟨10.1128/AAC.00148-10⟩, Antimicrobial Agents and Chemotherapy; Vol 54
- Publication Year :
- 2010
- Publisher :
- American Society for Microbiology, 2010.
-
Abstract
- Genotypic algorithms for prediction of HIV-1 coreceptor usage need to be evaluated in a clinical setting. We aimed at studying (i) the correlation of genotypic prediction of coreceptor use in comparison with a phenotypic assay and (ii) the relationship between genotypic prediction of coreceptor use at baseline and the virological response (VR) to a therapy including maraviroc (MVC). Antiretroviral-experienced patients were included in the MVC Expanded Access Program if they had an R5 screening result with Trofile (Monogram Biosciences). V3 loop sequences were determined at screening, and coreceptor use was predicted using 13 genotypic algorithms or combinations of algorithms. Genotypic predictions were compared to Trofile; dual or mixed (D/M) variants were considered as X4 variants. Both genotypic and phenotypic results were obtained for 189 patients at screening, with 54 isolates scored as X4 or D/M and 135 scored as R5 with Trofile. The highest sensitivity (59.3%) for detection of X4 was obtained with the Geno2pheno algorithm, with a false-positive rate set up at 10% (Geno2pheno10). In the 112 patients receiving MVC, a plasma viral RNA load of
- Subjects :
- Male
Oncology
MESH: Sequence Analysis, DNA
Multivariate analysis
HIV Infections
HIV Envelope Protein gp120
MESH: Receptors, CCR5
Maraviroc
MESH: Genotype
MESH: HIV-1
Correlation
MESH: HIV Envelope Protein gp120
chemistry.chemical_compound
HIV Protease
HIV Fusion Inhibitors
Genotype
MESH: Receptors, HIV
Pharmacology (medical)
MESH: Treatment Outcome
0303 health sciences
MESH: Middle Aged
MESH: Tropism
MESH: HIV Infections
Middle Aged
MESH: Cyclohexanes
HIV Reverse Transcriptase
3. Good health
Phenotype
Treatment Outcome
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Infectious Diseases
CCR5 Receptor Antagonists
Lentivirus
Female
Viral load
Adult
medicine.medical_specialty
Receptors, CCR5
MESH: HIV Reverse Transcriptase
Molecular Sequence Data
CCR5 receptor antagonist
Biology
MESH: Phenotype
Antiviral Agents
Sensitivity and Specificity
Tropism
MESH: HIV Protease
03 medical and health sciences
Receptors, HIV
Cyclohexanes
Internal medicine
medicine
Humans
MESH: HIV Fusion Inhibitors
030304 developmental biology
Pharmacology
MESH: Humans
MESH: Molecular Sequence Data
030306 microbiology
MESH: Adult
Sequence Analysis, DNA
Triazoles
biology.organism_classification
Virology
MESH: Male
MESH: Sensitivity and Specificity
MESH: Triazoles
chemistry
Expanded access
HIV-1
MESH: Female
Subjects
Details
- ISSN :
- 10986596 and 00664804
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- Antimicrobial Agents and Chemotherapy
- Accession number :
- edsair.doi.dedup.....4c57178d99db6ebdc6ec5ac609e7363c