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X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning

X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning

Authors :
Si Chen
Junjing Deng
Tom Peterka
Qiaoling Jin
David Vine
Youssef S. G. Nashed
Stefan Vogt
Chris Jacobsen
Source :
Scientific reports, vol 7, iss 1, Deng, J; Vine, DJ; Chen, S; Jin, Q; Nashed, YSG; Peterka, T; et al.(2017). X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning. Scientific Reports, 7(1). doi: 10.1038/s41598-017-00569-y. Lawrence Berkeley National Laboratory: Lawrence Berkeley National Laboratory. Retrieved from: http://www.escholarship.org/uc/item/5vd4k7fb, Scientific Reports, Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
Publication Year :
2017
Publisher :
Springer Science and Business Media LLC, 2017.

Abstract

X-ray microscopy can be used to image whole, unsectioned cells in their native hydrated state. It complements the higher resolution of electron microscopy for submicrometer thick specimens, and the molecule-specific imaging capabilites of fluorescence light microscopy. We describe here the first use of fast, continuous x-ray scanning of frozen hydrated cells for simultaneous sub-20 nm resolution ptychographic transmission imaging with high contrast, and sub-100 nm resolution deconvolved x-ray fluorescence imaging of diffusible and bound ions at native concentrations, without the need to add specific labels. By working with cells that have been rapidly frozen without the use of chemical fixatives, and imaging them under cryogenic conditions, we are able to obtain images with well preserved structural and chemical composition, and sufficient stability against radiation damage to allow for multiple images to be obtained with no observable change.

Details

ISSN :
20452322
Volume :
7
Database :
OpenAIRE
Journal :
Scientific Reports
Accession number :
edsair.doi.dedup.....4cdd9fde680cd3777e711e34decd7a50
Full Text :
https://doi.org/10.1038/s41598-017-00569-y