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X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning
X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning
- Source :
- Scientific reports, vol 7, iss 1, Deng, J; Vine, DJ; Chen, S; Jin, Q; Nashed, YSG; Peterka, T; et al.(2017). X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning. Scientific Reports, 7(1). doi: 10.1038/s41598-017-00569-y. Lawrence Berkeley National Laboratory: Lawrence Berkeley National Laboratory. Retrieved from: http://www.escholarship.org/uc/item/5vd4k7fb, Scientific Reports, Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- X-ray microscopy can be used to image whole, unsectioned cells in their native hydrated state. It complements the higher resolution of electron microscopy for submicrometer thick specimens, and the molecule-specific imaging capabilites of fluorescence light microscopy. We describe here the first use of fast, continuous x-ray scanning of frozen hydrated cells for simultaneous sub-20 nm resolution ptychographic transmission imaging with high contrast, and sub-100 nm resolution deconvolved x-ray fluorescence imaging of diffusible and bound ions at native concentrations, without the need to add specific labels. By working with cells that have been rapidly frozen without the use of chemical fixatives, and imaging them under cryogenic conditions, we are able to obtain images with well preserved structural and chemical composition, and sufficient stability against radiation damage to allow for multiple images to be obtained with no observable change.
- Subjects :
- 0301 basic medicine
Fluorescence-lifetime imaging microscopy
Materials science
Science
Image Processing
Analytical chemistry
Bioengineering
Article
Fluorescence
law.invention
03 medical and health sciences
Computer-Assisted
law
Freezing
Microscopy
Image Processing, Computer-Assisted
Radiation damage
Fluorescence microscope
Multidisciplinary
X-Rays
Chlamydomonas
Resolution (electron density)
Scanning confocal electron microscopy
X-ray
Water
Other Physical Sciences
030104 developmental biology
Microscopy, Fluorescence
Medicine
Biochemistry and Cell Biology
Generic health relevance
Electron microscope
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....4cdd9fde680cd3777e711e34decd7a50
- Full Text :
- https://doi.org/10.1038/s41598-017-00569-y