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The Application of Non-Invasive Apoptosis Detection Sensor (NIADS) on Histone Deacetylation Inhibitor (HDACi)-Induced Breast Cancer Cell Death

Authors :
Chia Hwa Lee
Po Li Wei
Chien Yu Huang
Ching Ling Lin
Wei Ming Chi
Kai Wen Hsu
Ka Wai Tam
Shou Tung Chen
Li Chi Huang
Yu Jia Chang
Chun-Yu Lin
Wen Shyang Hsieh
Source :
International Journal of Molecular Sciences, Vol 19, Iss 2, p 452 (2018), International Journal of Molecular Sciences, International Journal of Molecular Sciences; Volume 19; Issue 2; Pages: 452
Publication Year :
2018
Publisher :
MDPI AG, 2018.

Abstract

Breast cancer is the most common malignancy in women and the second leading cause of cancer death in women. Triple negative breast cancer (TNBC) subtype is a breast cancer subset without ER (estrogen receptor), PR (progesterone receptor) and HER2 (human epidermal growth factor receptor 2) expression, limiting treatment options and presenting a poorer survival rate. Thus, we investigated whether histone deacetylation inhibitor (HDACi) could be used as potential anti-cancer therapy on breast cancer cells. In this study, we found TNBC and HER2-enriched breast cancers are extremely sensitive to Panobinostat, Belinostat of HDACi via experiments of cell viability assay, apoptotic marker identification and flow cytometry measurement. On the other hand, we developed a bioluminescence-based live cell non-invasive apoptosis detection sensor (NIADS) detection system to evaluate the quantitative and kinetic analyses of apoptotic cell death by HDAC treatment on breast cancer cells. In addition, the use of HDACi may also contribute a synergic anti-cancer effect with co-treatment of chemotherapeutic agent such as doxorubicin on TNBC cells (MDA-MB-231), but not in breast normal epithelia cells (MCF-10A), providing therapeutic benefits against breast tumor in the clinic.

Details

ISSN :
14220067
Volume :
19
Database :
OpenAIRE
Journal :
International Journal of Molecular Sciences
Accession number :
edsair.doi.dedup.....4cddd14a643166461a69da17f522c593