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Bisbibenzyls, novel proteasome inhibitors, suppress androgen receptor transcriptional activity and expression accompanied by activation of autophagy in prostate cancer LNCaP cells
- Source :
- Pharmaceutical biology. 54(2)
- Publication Year :
- 2015
-
Abstract
- Bisbibenzyl compounds have gained our interests for their potential antitumor activity in malignant cell-types.The objective of this study is to investigate the effect of bisbibenzyl compounds riccardin C (RC), marchantin M (MM), and riccardin D (RD) on androgen receptor (AR) in prostate cancer (PCa) cells.After exposure to 10 μM of the compounds for 24 h, cell cycle and cell survival analyses were performed using FACS and MTT assay to confirm the effect of these bisbibenzyls on PCa LNCaP cells. Changes in the AR expression and function, as the result of exposure to the compounds, were investigated using real-time PCR, ELISA, transient transfection, western blotting (WB), immunoprecipitation, and immunofluorescence staining (IF). Chemical-induced autophagy was examined by WB, IF, and RNAi.RC, MM, and RD reduced the viability of LNCaP cells accompanied with arrested cell cycle in the G0/G1 phase and induction of apoptosis. Further investigation revealed that these compounds significantly inhibited AR expression at mRNA and protein levels, leading to the suppression of AR transcriptional activity. Moreover, inhibition of proteasome activity by bisbibenzyls, which in turn caused the induction of autophagy, as noted by induction of LC3B expression, conversion, and accumulation of punctate dots in treated cells. Co-localization of AR/LC3B and AR/Ub suggested that autophagy contributed to the degradation of polyubiquitinated-AR when proteasome activity was suppressed by the bisbibenzyls.Suppression of proteasome activity and induction of autophagy were involved in bisbibenzyl-mediated modulation of AR activities and apoptosis, suggesting their potential in treating PCa.
- Subjects :
- 0301 basic medicine
Hepatophyta
Male
Transcription, Genetic
Cell Survival
Pharmaceutical Science
Gene Expression
Apoptosis
Biology
03 medical and health sciences
Prostate cancer
0302 clinical medicine
Ethers, Cyclic
Cell Line, Tumor
Drug Discovery
LNCaP
Bibenzyls
Stilbenes
medicine
Autophagy
Humans
MTT assay
Pharmacology
Phenyl Ethers
Prostatic Neoplasms
General Medicine
Cell Cycle Checkpoints
Cell cycle
medicine.disease
Molecular biology
Antineoplastic Agents, Phytogenic
Androgen receptor
Blot
Protein Transport
030104 developmental biology
Complementary and alternative medicine
Receptors, Androgen
030220 oncology & carcinogenesis
Molecular Medicine
Proteasome Inhibitors
Subjects
Details
- ISSN :
- 17445116
- Volume :
- 54
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Pharmaceutical biology
- Accession number :
- edsair.doi.dedup.....4d07c59fb11d0ef1d0177d0946650dfd