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Pesticide treatment reduces hydrophobic pollutant contamination in Cucurbita pepo through competitive binding to major latex-like proteins
- Source :
- Environmental pollution (Barking, Essex : 1987). 266(Pt 2)
- Publication Year :
- 2020
-
Abstract
- Hydrophobic pollutants are still present in agricultural soil. The Cucurbitaceae family accumulates hydrophobic pollutants through roots, resulting in the contamination of aerial parts. Major latex-like proteins (MLPs), found in the Cucurbitaceae family, play an important role in the contamination by binding to these hydrophobic pollutants. Thus far, efficient cultivation methods for the production of safe crops with lower concentrations of hydrophobic pollutants have not been developed. Herein, we competitively inhibited the binding of MLPs to hydrophobic pollutants, pyrene and dieldrin, in roots by using MLP binding pesticides. By conducting a chemical array screening, we found that MLPs bound compounds with indole- and quinazoline-like structures. Commercially available pesticides amisulbrom and pyrifluquinazon, which possess such structures, successfully inhibited the binding of MLPs to pyrene and dieldrin in vitro. When zucchini plants were cultivated in the contaminated soil with 1.25 mmol/kg pyrene and 12.5 μmol/kg dieldrin, the concentration of pyrene and dieldrin in xylem sap was significantly decreased by 30% and 15%, respectively. Our results demonstrate that the pesticides binding to MLPs competitively inhibited the binding of MLPs to pyrene and dieldrin in roots, resulting in the reduction of overall contamination. This study proposes a novel approach to cultivate safer crops and advances the utilization of unknown functions of pesticides.
- Subjects :
- 010504 meteorology & atmospheric sciences
Latex
Health, Toxicology and Mutagenesis
Competitive binding inhibition
Hydrophobic pollutant
010501 environmental sciences
Toxicology
01 natural sciences
Binding, Competitive
Plant Roots
chemistry.chemical_compound
Dieldrin
Cucurbita pepo
Cucurbita
Major latex-like protein
Soil Pollutants
Pesticides
0105 earth and related environmental sciences
Pollutant
biology
General Medicine
Contamination
Pesticide
biology.organism_classification
Pollution
Soil contamination
chemistry
Environmental chemistry
Pyrene
Environmental Pollutants
Cucurbitaceae
Subjects
Details
- ISSN :
- 18736424
- Volume :
- 266
- Issue :
- Pt 2
- Database :
- OpenAIRE
- Journal :
- Environmental pollution (Barking, Essex : 1987)
- Accession number :
- edsair.doi.dedup.....4d08449fc14136c5a4f4bbc4d1259bb5