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Preferential 5-Methylcytosine Oxidation in the Linker Region of Reconstituted Positioned Nucleosomes by Tet1 Protein
- Source :
- Chemistry - A European Journal. 22:16598-16601
- Publication Year :
- 2016
- Publisher :
- Wiley, 2016.
-
Abstract
- Tet (ten-eleven translocation) family proteins oxidize 5-methylcytosine (mC) to 5-hydroxymethylcytosine (hmC), 5-formylcytosine (fC), and 5-carboxycytosine (caC), and are suggested to be involved in the active DNA demethylation pathway. In this study, we reconstituted positioned mononucleosomes using CpG-methylated 382 bp DNA containing the Widom 601 sequence and recombinant histone octamer, and subjected the nucleosome to treatment with Tet1 protein. The sites of oxidized methylcytosine were identified by bisulfite sequencing. We found that, for the oxidation reaction, Tet1 protein prefers mCs located in the linker region of the nucleosome compared with those located in the core region.
- Subjects :
- 0301 basic medicine
Stereochemistry
Catalysis
law.invention
Cytosine
03 medical and health sciences
chemistry.chemical_compound
law
Nucleosome
AFM imaging
Histone octamer
Organic Chemistry
DNA
General Chemistry
DNA Methylation
Linker DNA
proteins
Nucleosomes
5-Methylcytosine
030104 developmental biology
DNA demethylation
Biochemistry
chemistry
Recombinant DNA
Oxidation-Reduction
Linker
Subjects
Details
- ISSN :
- 09476539
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Chemistry - A European Journal
- Accession number :
- edsair.doi.dedup.....4d29e30c0c9dbd3973e8eb0484931a99