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In situ hybridization histochemistry of vgf mRNA in the rat suprachiasmatic nucleus: co-localization with vasopressin/neurophysin and VIP/PHI
- Source :
- Neuroscience letters. 189(1)
- Publication Year :
- 1995
-
Abstract
- The expression of vgf gene, first isolated as a gene induced by nerve growth factor in PC12 cells, was investigated in neurons of the suprachiasmatic nucleus (SCN) by in situ hybridization. In the rat forebrain, the vgf mRNA was found most densely in the SCN. Neurons which express vgf mRNA were found both in the dorsomedial and ventrolateral subdivisions. Soluble-labeling of vgf in situ hybridization and peptide immunocytochemistry demonstrated that vgf mRNA was expressed in most vasopressin- and neurophysin-immunoreactive neurons in the dorsomedial part and in vasoactive intestinal peptide (VIP)- and peptide histidine isoleucine amide (PHI)-immunoreactive neurons in the ventrolateral part. These findings suggest that vgf is a highly expressed gene in both vasopressin/neurophysin neurons and VIP/PHI neurons which were speculated to be involved in the generation and entrainment of circadian rhythm.
- Subjects :
- Male
medicine.medical_specialty
Vasopressin
Vasopressins
Vasoactive intestinal peptide
Neuropeptide
In situ hybridization
Neurophysins
Biology
Peptide PHI
Internal medicine
medicine
Animals
Tissue Distribution
RNA, Messenger
Rats, Wistar
In Situ Hybridization
Suprachiasmatic nucleus
General Neuroscience
Neuropeptides
Proteins
Immunohistochemistry
Cell biology
Rats
Endocrinology
nervous system
Hypothalamus
Suprachiasmatic Nucleus
hormones, hormone substitutes, and hormone antagonists
Vasoactive Intestinal Peptide
Subjects
Details
- ISSN :
- 03043940
- Volume :
- 189
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Neuroscience letters
- Accession number :
- edsair.doi.dedup.....4d3bd98ece2c20cc4158e7860397cfaf