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Self-targeting, zwitterionic micellar dispersants enhance antibiotic killing of infectious biofilms
- Source :
- Science Advances, Science Advances, 6(33):eabb1112. AMER ASSOC ADVANCEMENT SCIENCE
- Publication Year :
- 2020
-
Abstract
- Zwitterionic micelles find their own path through the body to disperse an infectious biofilm and enhance antibiotic efficacy.<br />Extracellular polymeric substances (EPS) hold infectious biofilms together and limit antimicrobial penetration and clinical infection control. Here, we present zwitterionic micelles as a previously unexplored, synthetic self-targeting dispersant. First, a pH-responsive poly(ε-caprolactone)-block-poly(quaternary-amino-ester) was synthesized and self-assembled with poly(ethylene glycol)-block-poly(ε-caprolactone) to form zwitterionic, mixed-shell polymeric micelles (ZW-MSPMs). In the acidic environment of staphylococcal biofilms, ZW-MSPMs became positively charged because of conversion of the zwitterionic poly(quaternary-amino-ester) to a cationic lactone ring. This allowed ZW-MSPMs to self-target, penetrate, and accumulate in staphylococcal biofilms in vitro. In vivo biofilm targeting by ZW-MSPMs was confirmed for staphylococcal biofilms grown underneath an implanted abdominal imaging window through direct imaging in living mice. ZW-MSPMs interacted strongly with important EPS components such as eDNA and protein to disperse biofilm and enhance ciprofloxacin efficacy toward remaining biofilm, both in vitro and in vivo. Zwitterionic micellar dispersants may aid infection control and enhance efficacy of existing antibiotics against remaining biofilm.
- Subjects :
- NANOCARRIERS
Intravital Microscopy
Polymers
02 engineering and technology
010402 general chemistry
medicine.disease_cause
01 natural sciences
Micelle
EXTRACELLULAR DNA
Mice
chemistry.chemical_compound
Extracellular polymeric substance
In vivo
medicine
NANOPARTICLES
Animals
DRUG-DELIVERY
Micelles
Research Articles
STAPHYLOCOCCUS-AUREUS
ARCHITECTURE
Multidisciplinary
NITRIC-OXIDE
Pseudomonas aeruginosa
Chemistry
Biofilm
PSEUDOMONAS-AERUGINOSA
SciAdv r-articles
biochemical phenomena, metabolism, and nutrition
021001 nanoscience & nanotechnology
Anti-Bacterial Agents
0104 chemical sciences
Applied Sciences and Engineering
Biofilms
Drug delivery
Biophysics
PENETRATION
Nanocarriers
0210 nano-technology
Ethylene glycol
RESISTANCE
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 23752548
- Volume :
- 6
- Issue :
- 33
- Database :
- OpenAIRE
- Journal :
- Science Advances
- Accession number :
- edsair.doi.dedup.....4d5078e70327b11462ead55ea4ee7be1