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Tuber melanosporum fermentation medium optimization by Plackett–Burman design coupled with Draper–Lin small composite design and desirability function
- Source :
- Bioresource Technology. 101:3139-3146
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- A novel method using Plackett-Burman design (PBD) coupled with Draper-Lin small composite design (SCD) and desirability function (DF) was developed to optimize Tuber melanosporum fermentation medium. Firstly, sucrose, yeast extract, peptone and Mg(2+) were identified to be key medium components by PBD. Secondly, in order to evaluate the relationships between responses and the identified key components, mathematical models were developed by SCD. Finally, by using DF, the responses were optimized simultaneously and the optimal concentration was located to be 73 g/L sucrose, 11 g/L yeast extract, 8 g/L peptone, and 46 mM Mg(2+). Under the identified optimal conditions, the maximal biomass, the production of extracellular (EPS) and intracellular (IPS) polysaccharides was 25.10 + or - 0.12 g/L, 3.88 + or - 0.23 g/L and 2.87 + or - 0.32 g/L, respectively, which agreed with the predicted values well. Compared with the basic medium, the biomass, the production of EPS and IPS was enhanced by 54.4%, 71.7% and 124.2%, respectively.
- Subjects :
- Environmental Engineering
Sucrose
Composite number
Intracellular Space
Bioengineering
Biology
Polysaccharide
Models, Biological
chemistry.chemical_compound
Polysaccharides
Botany
Yeast extract
Biomass
Food science
Waste Management and Disposal
chemistry.chemical_classification
Analysis of Variance
Plackett–Burman design
Renewable Energy, Sustainability and the Environment
Reproducibility of Results
General Medicine
Culture Media
Desirability function
chemistry
Tuber melanosporum
Pholiota
Fermentation
Regression Analysis
Extracellular Space
Subjects
Details
- ISSN :
- 09608524
- Volume :
- 101
- Database :
- OpenAIRE
- Journal :
- Bioresource Technology
- Accession number :
- edsair.doi.dedup.....4d5a06fddf6d8b536f6073a211854c23