Sensitive Voltammetric Sensor for Tryptophan Detection by Using Polyvinylpyrrolidone Functionalized Graphene/GCE

In this paper, an electrochemical method for the measurement of tryptophan (Trp) was developed based on a glassy carbon electrode modified with polyvinylpyrrolidonefunctionalized graphene (PVP-GR)/glassy carbon electrode (GCE). In 0.1 M phosphate buffer solution (PBS, pH = 2.2), compared with bare GCE, PVP/GCE, and GR/GCE, the oxidation peak current of Trp increased dramatically at PVP-GR/GCE. The oxidation mechanism of Trp on the PVP-GR/GCE was discussed and the experimental conditions were optimized. Under the best experimental conditions, the oxidation peak current of Trp was proportional to its concentration in the range of 0.06 &micro
M&ndash
10.0 &micro
M and 10.0&ndash
100.0 &micro
M, and the limit of detection (LOD) was 0.01 &micro
M (S/N = 3). The target modified electrode with excellent repeatability, stability and selectivity, was successfully applied to detectTrp in drugs and biological samples.