In vitro antibacterial activity of ethanol extract ofArtemisia annuaand its bioactive fractions against fish pathogens

Made available in DSpace on 2021-06-25T10:17:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-04-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Empresa Brasileira de Pesquisa Agropecuária Ministério da Agricultura, Pecuária e Abastecimento Bacterial pathogens cause significant economic losses in fish farms worldwide. The frequent use of antibiotics is a growing concern due to the adverse effects on human health and the environment. We evaluate the antibacterial activity of the Artemisia annua and its bioactive components against the Aeromonas hydrophila, Streptococcus agalactiae and Flavobacterium columnare, the main bacteria that affect fish culture. Bioactive components of the A. annua were obtained using an alcoholic extraction process, yielding 10.6% of dry material. The alcoholic extract of A. annua (ae-Aa) bioactive compounds was fractionated, obtaining the fractions F1 + 2, F3, F4, F5 and F6. Artemisinin up to 10.0 mg.ml-1 was ineffective in controlling studied bacteria. Microorganisms tested were all sensitive to ae-Aa (MIC and MBC 0.40 to 2.50 mg.ml-1). The ae-Aa components F3, F4, F5 and F6 were also effective (MIC and MBC 0.015 to 2.00 mg.ml-1). F3 showed the most effective antibacterial activity (MIC 0.015 to 0.5 mg.ml-1 and MBC 0.015 to 2.0 mg.ml-1). The ae-Aa and its bioactive components presented efficacy to inhibit growth and eliminate bacterial pathogens and can be an alternative to synthetic antibiotics for farmed fish. Joint Graduate Program in Physiological Sciences Federal University of São Carlos-UFSCar/São Paulo State University UNESP Centro Universitário de Jaguariúna – UniFAJ Brazilian Agricultural Research Corporation - Embrapa Environment (EMBRAPA) Chemical Biological and Agricultural Pluridisciplinary Research Center (CPQBA) Campinas State University (UNICAMP) Joint Graduate Program in Physiological Sciences Federal University of São Carlos-UFSCar/São Paulo State University UNESP