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Lipid profiles and differential lipids in serum related to severity of community-acquired pneumonia: A pilot study
- Source :
- PLoS ONE, Vol 16, Iss 3, p e0245770 (2021), PLoS ONE
- Publication Year :
- 2021
- Publisher :
- Public Library of Science (PLoS), 2021.
-
Abstract
- This study aimed to characterize the lipidomic responses to community-acquired pneumonia (CAP) and provide new insight into the underlying mechanisms of pathogenesis and potential avenues for diagnostic and therapeutic treatments. This study was performed from January 2017 to October 2018. Lipidomic profiles were generated using ultra high-performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS) platform. Spearman’s rank correlation test and multiple linear regression analysis were applied to explore the correlation between changes in the relative abundance of lipids and clinical parameters. Kaplan–Meier methods were used to build 30-day survival curves. From the UHPLC-MS/MS results, a total of 509 and 195 lipid species were detected in the positive and negative ionization mode respectively. Positive ionization covered six lipid classes (glycerol-phospholipids, glycerolipids, sphingolipids, sterol-lipids, prenol-lipids, and fatty acid), whilst negative ionization covered three (glycerol-phospholipids, sphingolipids, fatty acid). Four lipids were selected as targets: PC (16:0_18:1), PC (18:2_20:4), PC (36:4), and PC (38:6). The relative increase of the areas under the curves for all four lipids were superior to the pneumonia severity index and CURB-65 (confusion, urea, respiratory rate, blood pressure, and age ≥65 years old) for discriminating severe CAP from CAP. Decreasing relative levels of PC (18:2_20:4), PC (38:6), and PC (36:4) were negatively related to fraction of inspiration O2; Changes in the relative abundance of PC (16:0_18:1) and PC (18:2_20:4) had significantly linear relationship with procalcitonin. Patients with an elevated level of PC (16:0_18:1) had significantly longer duration of hospital stays. As the relative abundance of PC (18:2_20:4), PC (36:4), and PC (38:6) decreased, the length of hospitalization days and 30-day mortality rate increased significantly (all log-rank p
- Subjects :
- Male
0301 basic medicine
Pulmonology
Physiology
Pneumonia severity index
Pilot Projects
Biochemistry
Procalcitonin
0302 clinical medicine
Community-acquired pneumonia
Macromolecular Structure Analysis
Medicine and Health Sciences
chemistry.chemical_classification
Lipid Analysis
Multidisciplinary
Respiration
Lipids
Lipid Profiles
Community-Acquired Infections
Physical Sciences
Medicine
Female
Research Article
medicine.medical_specialty
Permutation
Science
03 medical and health sciences
Internal medicine
Lipid Structure
medicine
Humans
Molecular Biology
Survival analysis
Aged
Rank correlation
Discrete Mathematics
Biology and Life Sciences
Fatty acid
030208 emergency & critical care medicine
Lipid metabolism
Pneumonia
Lipid Metabolism
medicine.disease
Sphingolipid
Metabolism
030104 developmental biology
Endocrinology
chemistry
Combinatorics
Lipidomics
Physiological Processes
Mathematics
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 16
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....4dc0cedf46adf9ec20781e5cb2ef3b6e