Back to Search Start Over

Functional Gene Polymorphism to Reveal Species History: The Case of the CRTISO Gene in Cultivated Carrots

Authors :
Mathilde Briard
Didier Peltier
Emmanuel Geoffriau
Matthieu Jourdan
Sébastien Huet
Vanessa Soufflet-Freslon
Jérémy Clotault
Génétique et Horticulture (GenHort)
AGROCAMPUS OUEST
Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)
Génétique Diversité et Ecophysiologie des Céréales (GDEC)
Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP)
Institut de Recherche en Horticulture et Semences (IRHS)
Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST
Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
French Ministry of Research
French Ministry of Agriculture (DGER)
Ministry of Agriculture (DGER)
Ministry of Research
Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST
Source :
PLoS ONE, Plos One 8 (8), . (2013), PLoS ONE, Public Library of Science, 2013, 8 (8), pp.e70801. ⟨10.1371/journal.pone.0070801⟩, PLoS ONE, 2013, 8 (8), pp.e70801. ⟨10.1371/journal.pone.0070801⟩, PLoS ONE, Vol 8, Iss 8, p e70801 (2013)
Publication Year :
2013
Publisher :
Public Library of Science (PLoS), 2013.

Abstract

International audience; BACKGROUND: Carrot is a vegetable cultivated worldwide for the consumption of its root. Historical data indicate that root colour has been differentially selected over time and according to geographical areas. Root pigmentation depends on the relative proportion of different carotenoids for the white, yellow, orange and red types but only internally for the purple one. The genetic control for root carotenoid content might be partially associated with carotenoid biosynthetic genes. Carotenoid isomerase (CRTISO) has emerged as a regulatory step in the carotenoid biosynthesis pathway and could be a good candidate to show how a metabolic pathway gene reflects a species genetic history. METHODOLOGY/PRINCIPAL FINDINGS: In this study, the nucleotide polymorphism and the linkage disequilibrium among the complete CRTISO sequence, and the deviation from neutral expectation were analysed by considering population subdivision revealed with 17 microsatellite markers. A sample of 39 accessions, which represented different geographical origins and root colours, was used. Cultivated carrot was divided into two genetic groups: one from Middle East and Asia (Eastern group), and another one mainly from Europe (Western group). The Western and Eastern genetic groups were suggested to be differentially affected by selection: a signature of balancing selection was detected within the first group whereas the second one showed no selection. A focus on orange-rooted carrots revealed that cultivars cultivated in Asia were mainly assigned to the Western group but showed CRTISO haplotypes common to Eastern carrots. CONCLUSION: The carotenoid pathway CRTISO gene data proved to be complementary to neutral markers in order to bring critical insight in the cultivated carrot history. We confirmed the occurrence of two migration events since domestication. Our results showed a European background in material from Japan and Central Asia. While confirming the introduction of European carrots in Japanese resources, the history of Central Asia material remains unclear.

Details

ISSN :
19326203
Volume :
8
Database :
OpenAIRE
Journal :
PLoS ONE
Accession number :
edsair.doi.dedup.....4ddda79afe9ce6f0412a1ccbb0e21b23
Full Text :
https://doi.org/10.1371/journal.pone.0070801