Back to Search
Start Over
The Action of the First Component of Complement on Cells Sensitized with Blood Group Antibody
- Source :
- British Journal of Haematology. 11:592-599
- Publication Year :
- 1965
- Publisher :
- Wiley, 1965.
-
Abstract
- We have always experienced difficulties in the past in treating cells sensitized with blood group antibody with the first component of complement so that they became strongly active following such treatment. Such cells, prepared as described by other workers (Harboe, Muller-Eberhard, Fudenberg, Polley and Mollison, 1963) have always proved to be insufficiently active for our experiments. We therefore explored a number of alternative methods, and found that a pellet obtained by ultracentrifugation of human serum (Naff, Pensky and Lepow, 1964) could be used for this purpose. The object of this paper is to describe the treatment of cells sensitized with blood group antibodies with this pellet and to describe the reactions of the cells so prepared.
- Subjects :
- Alternative methods
Complement (group theory)
Blood Cells
biology
Chemistry
Immune Sera
Gamma globulin
Complement System Proteins
Hematology
In Vitro Techniques
Immune sera
Antibodies
Antibodies, Anti-Idiotypic
On cells
Blood group antibodies
Immunology
Blood Group Antigens
biology.protein
Humans
Indicators and Reagents
Component (group theory)
gamma-Globulins
Antibody
Ultracentrifugation
Subjects
Details
- ISSN :
- 13652141 and 00071048
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- British Journal of Haematology
- Accession number :
- edsair.doi.dedup.....4e4d4155af89738add692e25e2215e6a