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Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis
- Source :
- Journal of visualized experiments : JoVE, Journal of visualized experiments : JoVE, 2021, 168, ⟨10.3791/61133⟩
- Publication Year :
- 2021
- Publisher :
- MyJove Corporation, 2021.
-
Abstract
- International audience; Methods for virus particle quantification represent a critical aspect of many virology studies. Although several reliable techniques exist, they are either time-consuming or unable to detect small variations. Presented here is a protocol for the precise quantification of viral titer by analyzing electrical impedance variations of infected cells in real-time. Cellular impedance is measured through gold microelectrode biosensors located under the cells in microplates, in which magnitude depends on the number of cells as well as their size and shape. This protocol allows real-time analysis of cell proliferation, viability, morphology and migration with enhanced sensitivity. Also provided is an example of a practical application by quantifying the decay of influenza A virus (IAV) submitted to various physicochemical parameters affecting viral infectivity over time (i.e., temperature, salinity, and pH). For such applications, the protocol reduces the workload needed while also generating precise quantification data of infectious virus particles. It allows the comparison of inactivation slopes among different IAV, which reflects their capacity to persist in given environment. This protocol is easy to perform, is highly reproducible, and can be applied to any virus producing cytopathic effects in cell culture.
- Subjects :
- [SDV]Life Sciences [q-bio]
General Chemical Engineering
Cell analysis
Hemagglutinin Glycoproteins, Influenza Virus
Biology
medicine.disease_cause
Virus
General Biochemistry, Genetics and Molecular Biology
Madin Darby Canine Kidney Cells
Dogs
Computer Systems
Influenza A virus
medicine
Animals
Infectious virus
Infectivity
Microbial Viability
General Immunology and Microbiology
Host (biology)
Cell growth
General Neuroscience
Viral Load
Cell biology
Titer
Kinetics
Mutation
Linear Models
Virus Inactivation
Subjects
Details
- ISSN :
- 1940087X
- Database :
- OpenAIRE
- Journal :
- Journal of Visualized Experiments
- Accession number :
- edsair.doi.dedup.....4ea5673d7ac18367cdc3229bb967423f