Supp Fig S1-S5 and Supp Tables S1-S2 from CD271 Confers an Invasive and Metastatic Phenotype of Head and Neck Squamous Cell Carcinoma through the Upregulation of Slug

Supplementary Figure S1. Comparisons of mRNA expressions of EMTrelated genes (Vimentin, CDH1, CDH2, Twist1, TGFß1) between MOC2 vs MOC2-CD271 cells, and between HSC3 vs HSC3-CD271 cells by qRT-PCR; Supplementary Figure S2. Comparisons of mRNA expression of ligands (NGF, BDNF, NT-4) for the CD271 receptor between CD45+ cells, stromal cells and tumors by qRTPCR; Supplementary Figure S3. Representative photomicrographs of morphologic changes of murine or human cancer cells (MOC2-CD271, HSC3-CD271, respectively) in response to rhNGF-β treatment for 48 hrs; Supplementary Figure S4. CD271 activation induces Snai2 expression through the ERK1/2 pathway; Supplementary figure S5. Quantification methods for CD271 expression in primary oral tumor by immunohistochemistry using Photoshop and Image J; Supplementary Table 1. Profiles of patients with oral squamous cell carcinoma analyzed by immunohistochemistry CD271 expression; Supplemental Table 2. Cox proportional hazard model to identify predictors of disease-free survival in pN+ patients.