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Kuguacin J, a triterpeniod from Momordica charantia leaf, modulates the progression of androgen-independent human prostate cancer cell line, PC3
Kuguacin J, a triterpeniod from Momordica charantia leaf, modulates the progression of androgen-independent human prostate cancer cell line, PC3
- Source :
- Food and Chemical Toxicology. 50:840-847
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- In this study, we focused on the in vitro effects of Kuguacin J (KuJ), a purified component of bitter melon (Momordica charantia) leaf extract (BMLE), on the androgen-independent human prostate cancer cell line PC3 and the in vivo effect of dietary BMLE on prostate carcinogenesis using a PC3-xenograph model. KuJ exerted a strong growth-inhibitory effect on PC3 cells. Growth inhibition was mainly through G1-arrest: KuJ markedly decreased the levels of cyclins (D1 and E), cyclin-dependent kinases (Cdk2 and Cdk4) and proliferating cell nuclear antigen. Interestingly, KuJ also dramatically decreased the levels of survivin expressed by PC3 cells. In addition, KuJ exerted anti-invasive effects on PC3 cells, significantly inhibiting migration and invasion: KuJ inhibited secretion of the active forms of MMP-2, MMP-9 and uPA by PC3 cells. In addition, KuJ treatment significantly decreased the expression of membrane type 1-MMP (MT1-MMP) by PC3 cells. In vivo, 1% and 5% BMLE in the diet resulted in 63% and 57% inhibition of PC3 xenograft growth without adverse effect on host body weight. Our results suggest that KuJ is a promising new candidate chemopreventive and chemotherapeutic agent for prostate cancer.
- Subjects :
- Male
Blotting, Western
Mice, Nude
Apoptosis
Real-Time Polymerase Chain Reaction
urologic and male genital diseases
Toxicology
Mice
chemistry.chemical_compound
Prostate cancer
In vivo
Cell Line, Tumor
Survivin
In Situ Nick-End Labeling
medicine
Animals
Humans
Momordica
RNA, Messenger
neoplasms
DNA Primers
Cyclin
Base Sequence
biology
Cell growth
Kinase
Cell Cycle
Prostatic Neoplasms
General Medicine
medicine.disease
Xenograft Model Antitumor Assays
Matrix Metalloproteinases
Triterpenes
Proliferating cell nuclear antigen
Plant Leaves
chemistry
Biochemistry
Androgens
Disease Progression
Cancer research
biology.protein
Growth inhibition
Food Science
Subjects
Details
- ISSN :
- 02786915
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- Food and Chemical Toxicology
- Accession number :
- edsair.doi.dedup.....4ee3b253d2adc88116579f97c72dc6fd
- Full Text :
- https://doi.org/10.1016/j.fct.2012.01.009