Additional file 1 of The first report on the sortase-mediated display of bioactive protein A from Staphylococcus aureus (SpA) on the surface of the vegetative form of Bacillus subtilis

Additional file 1: Figure S1. pChiM is a plasmid containing cell wall sorting motifs of B. subtilis (CWM), sortase cleavage motif (LPDTS) and the enzyme sortase (YhcS). This plasmid was used as a template for making other constructs prepared in this study. Figure S2. Schematic representation of SpA display constructs. Plasmids were constructed to express the SpA with or without the C-terminal cell wall motif (CWM) of YhcR, and with or without the sortase YhcS. a pSpA-YhcS is the main construct for SpA display on the B. subtilis cell wall surface, b the control construct named pNC1 lacks the sortase, c the control construct named pNC2 lacks both the CWM and sortase. SD Shine Dalgarno sequence; spa S. aureus protein A; CWM cell wall motif; YhcS sortase of B. subtilis. Figure S3. Diagrammatic representation of the constructs used in this study as control plasmids (pNC1 and pNC2) and main construct (pSpA-YhcS). Figure S4. The process of making constructs and their transformation. Table S1. Oligonucleotide primers sequences used in this study.