Back to Search
Start Over
Ectopic expression of the AaFUL1 gene identified in Anthurium andraeanum affected floral organ development and seed fertility in tobacco
- Source :
- Gene. 696:197-205
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Anthurium andraeanum is a high-grade potted flower that enjoys global popularity. Its floral organs have been substantially modified, and its ornamental value is based on its petaloid bracts. MADS-box gene products are important transcription factors that control plant development. In particular, the APETALA1 (AP1)/FRUITFULL (FUL) family of MADS-box genes plays a key role in flowering transitions and out-whorl floral organ identity specification. In this report, one FUL-like gene was cloned from Anthurium andraeanum and named AaFUL1 after bioinformatics identification. Subsequent subcellular localization experiments confirmed that the AaFUL1 protein was located in the nucleus, and data obtained from an expression analysis indicated that the relative expression level of AaFUL1 was the highest in bracts and inflorescences, while its expression was relatively low in stems and roots. Next, an AaFUL1 overexpression vector was constructed and ectopically expressed in tobacco. The transformants did not show any early flowering phenotype, but the average internode length of the inflorescence branch was significantly higher than that observed in the control, and its petal color had substantially faded. The morphology of the petal and pistil was clearly changed, the fruit was deformed, and the seed was largely aborted. These data indicate that even though the sequence of AaFUL1 is relatively conserved, its function differs from that of other orthologs, and the FUL subfamily of MADS-box transcription factors may have taken on new functions during the evolution processes. The results of this experiment enrich our knowledge of FUL transcription factors in monocotyledon plants.
- Subjects :
- 0301 basic medicine
Gynoecium
MADS Domain Proteins
Flowers
Genes, Plant
Monocotyledon
Ectopic Gene Expression
Evolution, Molecular
03 medical and health sciences
0302 clinical medicine
Gene Expression Regulation, Plant
Tobacco
Botany
Genetics
Anthurium andraeanum
Araceae
Phylogeny
MADS-box
Plant Proteins
Bract
biology
fungi
Gene Expression Regulation, Developmental
food and beverages
General Medicine
Plants, Genetically Modified
biology.organism_classification
Fertility
Phenotype
030104 developmental biology
Inflorescence
030220 oncology & carcinogenesis
Seeds
Ectopic expression
Petal
Sequence Alignment
Subjects
Details
- ISSN :
- 03781119
- Volume :
- 696
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....4f38a342b82350448fd822f964d7351c