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Evaluation of ELISA kits for the screening of four nitrofuran metabolites in aquaculture products according to the European guideline for the validation of screening methods
- Source :
- Food additives & contaminants. Part A. Chemistry, analysis, control, exposure & risk assessment, Food additives & contaminants. Part A. Chemistry, analysis, control, exposure & risk assessment, Taylor & Francis, 2021, 38 (2), pp.237-254. ⟨10.1080/19440049.2020.1849821⟩
- Publication Year :
- 2020
-
Abstract
- International audience; The administration of nitrofurans to livestock to treat or prevent animal diseases has been banned in the EU for the production of food of animal origin. The corresponding marker residues are tissue-related metabolites AMOZ, AHD, SEM, and AOZ. The MRPL (minimum required performance limit)/RPA (Reference point for action) was set at 1 µg kg(-1) in the EU. Thus, all the laboratories involved in the control of nitrofuran metabolites must detect at least at this analytical limit of performance. The objectives of the work reported here were to evaluate the performance of ELISA kits from two different manufacturers (R-Biopharm, Germany; Europroxima, the Netherlands) for the individual screening of the four nitrofuran metabolites (AOZ, AMOZ; AHD; and SEM) in aquaculture products (fish, shrimps), and then to validate the kits according to the European Decision EC/2002/657 and to the European guideline for the validation of screening methods. The false positive rates were below 9 % for the kits from both manufacturers. The detection capabilities CCβ determined were all below the current RPA (1 µg/kg). However, regarding the updated RPA at 0.5 µg/kg that shall apply in 2022, the AMOZ and SEM kits from R-Biopharm and the SEM kit from Europroxima will not be able to reach it.
- Subjects :
- analysis
Nitrofurans
Health, Toxicology and Mutagenesis
analyse
Drug Evaluation, Preclinical
nitrofurane
veterinary drug
Aquaculture
Toxicology
01 natural sciences
Animal origin
residues
Screening method
kit ELISA
résidus
Nitrofuran
Fishes
04 agricultural and veterinary sciences
General Medicine
040401 food science
Europe
food safety
ELISA kit
Livestock
médicament vétérinaire
medicine.drug_class
Enzyme-Linked Immunosorbent Assay
Food Contamination
nitrofuran
chemistry
legislation
Elisa kit
0404 agricultural biotechnology
[CHIM.ANAL]Chemical Sciences/Analytical chemistry
Fish Products
medicine
Animals
14. Life underwater
European Union
sécurité des aliments
business.industry
010401 analytical chemistry
législation
Public Health, Environmental and Occupational Health
General Chemistry
Guideline
0104 chemical sciences
Biotechnology
business
Food Analysis
Food Science
Subjects
Details
- ISSN :
- 19440057 and 19440049
- Volume :
- 38
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Food additivescontaminants. Part A, Chemistry, analysis, control, exposurerisk assessment
- Accession number :
- edsair.doi.dedup.....4f5099fc14a835b4938c980246d47004
- Full Text :
- https://doi.org/10.1080/19440049.2020.1849821⟩