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Subcellular localization of glypican-5 is associated with dynamic motility of the human mesenchymal stem cell line U3DT
- Source :
- PLoS ONE, Vol 16, Iss 2, p e0226538 (2021), PLoS ONE
- Publication Year :
- 2021
- Publisher :
- Public Library of Science (PLoS), 2021.
-
Abstract
- Glypican-5 (GPC5) is a heparan sulfate proteoglycan (HSPG) localized to the plasma membrane. We previously reported that in the human mesenchymal stem cell line UE6E7T-3, GPC5 is overexpressed in association with transformation and promotes cell proliferation by acting as a co-receptor for Sonic hedgehog signaling. In this study, we found using immunofluorescence microscopy that in transformed cells (U3DT), GPC5 localized not only at primary cilia on the cell surface, but also at the leading edge of migrating cells, at the intercellular bridge and blebs during cytokinesis, and in extracellular vesicles. In each subcellular region, GPC5 colocalized with fibroblast growth factor receptor (FGFR) and the small GTPases Rab11 and ARF6, indicating that GPC5 is delivered to these regions by Rab11-associated recycling endosomes. These colocalizations suggest that GPC5 plays an important role in FGF2 stimulation of cell migration, which was abrogated by knockdown of GPC5. Our findings indicate that GPC5 plays a role in regulation of U3DT cell migration and provides several insights into the functions of GPC5 that could be elucidated by future studies.
- Subjects :
- Glypican
Cell Membranes
Immunofluorescence
Cell
Cell Signaling
Cell Movement
Cell Cycle and Cell Division
Staining
Multidisciplinary
Chemistry
Cell Staining
Cell migration
Hedgehog signaling pathway
Cell biology
Cell Motility
medicine.anatomical_structure
Cell Processes
Fibroblast growth factor receptor
Medicine
Cellular Structures and Organelles
Research Article
Signal Transduction
Imaging Techniques
Science
Cell Migration
Research and Analysis Methods
Glypicans
Cell Line, Tumor
Fluorescence Imaging
medicine
Humans
Hedgehog Proteins
Telophase
Immunoassays
Cytokinesis
Cell Proliferation
Cell growth
Cell Membrane
Mesenchymal stem cell
Biology and Life Sciences
Mesenchymal Stem Cells
Cell Biology
Receptors, Fibroblast Growth Factor
Specimen Preparation and Treatment
Immunologic Techniques
Hedgehog Signaling
Carrier Proteins
Heparan Sulfate Proteoglycans
Developmental Biology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....4f551c54d720eeb34a6312e3569d3c90