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LoTToR: An Algorithm for Missing-Wedge Correction of the Low-Tilt Tomographic 3D Reconstruction of a Single-Molecule Structure
- Source :
- Scientific reports, vol 10, iss 1, Scientific Reports, Vol 10, Iss 1, Pp 1-17 (2020), Scientific Reports
- Publication Year :
- 2020
- Publisher :
- eScholarship, University of California, 2020.
-
Abstract
- A single-molecule three-dimensional (3D) structure is essential for understanding the thermal vibrations and dynamics as well as the conformational changes during the chemical reaction of macromolecules. Individual-particle electron tomography (IPET) is an approach for obtaining a snap-shot 3D structure of an individual macromolecule particle by aligning the tilt series of electron tomographic (ET) images of a targeted particle through a focused iterative 3D reconstruction method. The method can reduce the influence on the 3D reconstruction from large-scale image distortion and deformation. Due to the mechanical tilt limitation, 3D reconstruction often contains missing-wedge artifacts, presented as elongation and an anisotropic resolution. Here, we report a post-processing method to correct the missing-wedge artifact. This low-tilt tomographic reconstruction (LoTToR) method contains a model-free iteration process under a set of constraints in real and reciprocal spaces. A proof of concept is conducted by using the LoTToR on a phantom, i.e., a simulated 3D reconstruction from a low-tilt series of images, including that within a tilt range of ±15°. The method is validated by using both negative-staining (NS) and cryo-electron tomography (cryo-ET) experimental data. A significantly reduced missing-wedge artifact verifies the capability of LoTToR, suggesting a new tool to support the future study of macromolecular dynamics, fluctuation and chemical activity from the viewpoint of single-molecule 3D structure determination.
- Subjects :
- 0301 basic medicine
Electron Microscope Tomography
Macromolecular Substances
Science
Image Processing
Molecular Conformation
Image processing
Bioengineering
Negative Staining
Article
Imaging
03 medical and health sciences
Imaging, Three-Dimensional
0302 clinical medicine
Computer-Assisted
Distortion
Image Processing, Computer-Assisted
Structure determination
Tomography
Physics
Artifact (error)
Multidisciplinary
Tomographic reconstruction
3D reconstruction
Cryoelectron Microscopy
X-Ray Computed
030104 developmental biology
Tilt (optics)
Electron tomography
Three-Dimensional
Cryoelectron tomography
Thermodynamics
Medicine
Biomedical Imaging
Tomography, X-Ray Computed
Artifacts
Algorithm
Software
030217 neurology & neurosurgery
Algorithms
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Scientific reports, vol 10, iss 1, Scientific Reports, Vol 10, Iss 1, Pp 1-17 (2020), Scientific Reports
- Accession number :
- edsair.doi.dedup.....4f6e467b467ea7a134fe24010a54e391