DNA metabarcoding of gut contents reveals key habitat and seasonal drivers of trophic networks involving generalist predators in agricultural landscapes

BACKGROUND: Understanding the networks of trophic interactions into which generalist predators are embedded is key to assessing their ecological role of in trophic networks and the biological control services they provide. The advent of affordable DNA metabarcoding approaches greatly facilitates quantitative understanding of trophic networks and their response to environmental drivers. Here, we examine how key environmental gradients interact to shape predation by Lycosidae in highly dynamic vegetable growing systems in China. RESULTS: For the sampled Lycosidae, crop identity, pesticide use and seasons shape the abundance of prey detected in spider guts. For the taxonomic richness of prey, local- and landscape-scale factors gradients were more influential. Multivariate ordinations confirm that these crop-abundant spiders dynamically adjust their diet to reflect environmental constraints and seasonal availability to prey. CONCLUSION: Plasticity in diet composition is likely to account for the persistence of spiders in relatively ephemeral brassica crops. Our findings provide further insights into the optimization of habitatmanagement for predator-based biological control practices. This work was financially supported by the National Natural Science Foundation of China (no. 31972271), State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Joint International Research Laboratory of Ecological Pest Control, Fujian-Taiwan Joint Innovation Centre for Ecological Control of Crop Pests, International science and technology cooperation and exchange program of FAFU (KXb16014A), and the Thousand Talents Program and the ‘111’ Program in China. info:eu-repo/semantics/publishedVersion