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Crebanine inhibits voltage-dependent Na+ current in guinea-pig ventricular myocytes
- Source :
- Chinese Journal of Natural Medicines. 12:20-23
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- Aim To study the effects of crebanine on voltage-gated Na + channels in cardiac tissues. Methods Single ventricular myocytes were enzymatically dissociated from adult guinea-pig heart. Voltage-dependent Na + current was recorded using the whole cell voltage-clamp technique. Results Crebanine reversibly inhibited Na + current with an IC 50 value of 0.283 mmol·L −1 (95% confidence range: 0.248–0.318 mmol·L −1 ). Crebanine at 0.262 mmol·L −1 caused a negative shift (about 12 mV) in the voltage-dependence of steady-state inactivation of Na + current, and retarded its recovery from inactivation, but did not affect its activation curve. Conclusion In addition to blocking other voltage-gated ion channels, crebanine blocked Na + channels in guinea-pig ventricular myocytes. Crebanine acted as an inactivation stabilizer of Na + channels in cardiac tissues.
- Subjects :
- Male
Aporphines
Heart Ventricles
Guinea Pigs
Down-Regulation
Voltage-Gated Sodium Channels
Na current
Guinea pig
Negative shift
Drug Discovery
Animals
Myocytes, Cardiac
Patch clamp
Ventricular myocytes
Cells, Cultured
Ion channel
Stephania
Voltage-Gated Sodium Channel Blockers
Chemistry
Sodium channel
General Medicine
Anatomy
Complementary and alternative medicine
Biophysics
Female
Whole cell
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 18755364
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Chinese Journal of Natural Medicines
- Accession number :
- edsair.doi.dedup.....4f8683c7c0b5363b5ea034d5951efef3