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Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma
- Source :
- Oncotarget
- Publication Year :
- 2015
- Publisher :
- Impact Journals, LLC, 2015.
-
Abstract
- The expression of annexin A2 (ANXA2) in nasopharyngeal carcinoma (NPC) cells induces the immunosuppressive response in dendritic cells; however, the oncogenic effect and clinical significance of ANXA2 have not been fully investigated in NPC cells. Immunohistochemical staining for ANXA2 was performed in 61 patients and the association with clinicopathological status was determined. Short hairpin (sh)RNA knockdown of ANXA2 was used to examine cellular effects of ANXA2, by investigating alterations in cell proliferation, migration, invasion, adhesion, tube-formation assay, and chemo- and radiosensitivity assays were performed. RT-qPCR, Western blotting, and immunofluorescence were applied to determine molecular expression levels. Clinical association studies showed that the expression of ANXA2 was significantly correlated with metastasis (p = 0.0326) and poor survival (p = 0.0256). Silencing of ANXA2 suppressed the abilities of cell proliferation, adhesion, migration, invasion, and vascular formation in NPC cell. ANXA2 up-regulated epithelial-mesenchymal transition associated signal proteins. Moreover, ANXA2 reduced sensitivities to irradiation and chemotherapeutic drugs. These results define ANXA2 as a novel prognostic factor for malignant processes, and it can serve as a molecular target of therapeutic interventions for NPC.
- Subjects :
- Adult
Male
Pathology
medicine.medical_specialty
Epithelial-Mesenchymal Transition
Cell
Nasopharyngeal neoplasm
Antineoplastic Agents
chemotherapy
Cell Movement
Cell Adhesion
Humans
Medicine
Gene silencing
Gene Silencing
Epithelial–mesenchymal transition
RNA, Small Interfering
Cell adhesion
Annexin A2
radiotherapy
Aged
Cell Proliferation
Aged, 80 and over
nasopharyngeal carcinoma (NPC)
Nasopharyngeal Carcinoma
business.industry
Cell growth
Carcinoma
annexin A2 (ANXA2)
Nasopharyngeal Neoplasms
Middle Aged
Prognosis
medicine.disease
Immunohistochemistry
epithelial-mesenchymal transition (EMT)
Cell Transformation, Neoplastic
Phenotype
medicine.anatomical_structure
Oncology
Nasopharyngeal carcinoma
Drug Resistance, Neoplasm
Cancer research
Female
business
Signal Transduction
Research Paper
Subjects
Details
- ISSN :
- 19492553
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Oncotarget
- Accession number :
- edsair.doi.dedup.....4fba147690a8432782e266e96c99cf39