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Development and evaluation of a minicolumn assay for the detection of aflatoxin M1 in milk
- Source :
- Journal of dairy science. 77(5)
- Publication Year :
- 1994
-
Abstract
- A practical field method for the chemiselective immobilization and detection of aflatoxin M 1 in milk has been developed and is being marketed. In this new method, aflatoxin M 1 is selectively adsorbed at the interface of a layer of neutral sand and a band of magnesium silicate (Florisil) packed in a glass minicolumn. Aflatoxin M 1 , at ≥.5 ppb in contaminated milk, can be easily visualized as a band of bright blue fluorescence. Briefly, raw or homogenized and pasteurized milk is diluted with water (1:1, vol/vol) and passed through a C 18 cartridge. Aflatoxin M 1 is then partitioned by polarity, eluted with acetone-methylene chloride, and added to the minicolumn. The minicolumn is washed and viewed under long wave UV light. The limit of detection for this assay was .2 ppb, which was similar to the .3 ppb obtained using an immunoaffinity column, followed by minicolumn detection. The assay was accurate, rapid, easy to perform, and stable.
- Subjects :
- Aflatoxin
Pasteurization
Food Contamination
Chloride
Chromatography, Affinity
law.invention
chemistry.chemical_compound
Cartridge
Adsorption
law
Magnesium Silicates
Genetics
medicine
Animals
Mycotoxin
Chromatography, High Pressure Liquid
Detection limit
Chromatography
Elution
Milk
chemistry
Aflatoxin M1
Animal Science and Zoology
Food Science
medicine.drug
Subjects
Details
- ISSN :
- 00220302
- Volume :
- 77
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Journal of dairy science
- Accession number :
- edsair.doi.dedup.....4fc496b616cd17a028eb126cfbaa7b78