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A colorimetric assay system for dopamine using microfluidic paper-based analytical devices
- Source :
- Talanta. 194:171-176
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- We report the colorimetric detection of dopamine (DA) on microfluidic paper-based analytical devices (μPADs) using an oxidation-reduction method. Here, dopamine reacts with ferric chloride forming reduced Fe2+ that subsequently reacts with phenanthroline to form the red tris(1,10-phenanthroline)iron(II) complex. The devices were fabricated by wax printing and changes in color intensity were recorded using a common cell phone. Subsequent analysis using Photoshop software, yielded a limit of detection (LOD) for DA of 0.37 μmol/L with a linear range of 0.527–4.75 μmol/L and relative standard deviation of 0.11% (inter-day) and 0.15% (intra-day) for n = 15 paper chips. The effects of detection conditions have been investigated and are discussed. Cow serum samples and human blood serum and plasma samples were detected. The work, herein, demonstrates the potential of this method as a low cost and rapid colorimetric technique to detect DA in real samples.
- Subjects :
- Paper
Time Factors
Dopamine
Iron
Phenanthroline
Microfluidics
02 engineering and technology
01 natural sciences
Chloride
Analytical Chemistry
chemistry.chemical_compound
Lab-On-A-Chip Devices
medicine
Humans
Colorimetry
Detection limit
Wax
Chromatography
010401 analytical chemistry
021001 nanoscience & nanotechnology
0104 chemical sciences
chemistry
Linear range
visual_art
Calibration
visual_art.visual_art_medium
Ferric
0210 nano-technology
Oxidation-Reduction
medicine.drug
Subjects
Details
- ISSN :
- 00399140
- Volume :
- 194
- Database :
- OpenAIRE
- Journal :
- Talanta
- Accession number :
- edsair.doi.dedup.....4fe39673a5c35f06bb543bd1ed875e41